Bárbara A A Porto1, Cinthia F Monteiro1, Éricka L S Souza1, Paola C L Leocádio2, Jacqueline I Alvarez-Leite2, Simone V Generoso3, Valbert N Cardoso4, Camila M Almeida-Leite5, Daniel A Santos1, Julliana R A Santos6, Jacques R Nicoli1, Enrica Pessione7, Flaviano S Martins8. 1. Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. 2. Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. 3. Department of Basic Nursing, School of Nursing, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. 4. Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. 5. Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. 6. Laboratory of Environmental Microbiology, CEUMA University (UNICEUMA), São Luís, MA, Brazil. 7. Life Science and Systems Biology Department, University of Torino, Torino, Italy. 8. Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. flaviano@icb.ufmg.br.
Abstract
PURPOSE: Gastrointestinal mucositis is a major problem associated with cancer therapy. To minimize these deleterious effects, simultaneous administration of antioxidant components, such as selenium, can be considered. There is a growing interest in the use of yeasts because they are able to convert inorganic selenium into selenomethionine. In the present study, oral administration of Saccharomyces cerevisiae UFMG A-905 enriched with selenium was evaluated as an alternative in minimizing the side effects of 5FU-induced mucositis in mice. METHODS: Mice body weight, food consumption, faeces consistency and the presence of blood in faeces were assessed daily during experimental mucositis induced by 5-fluorouracil (5FU). Blood was used for intestinal permeability determination, and small intestine for oxidative stress, immunological and histopathological examination. RESULTS: The increased intestinal permeability observed with mucositis induction was partially reverted by S. cerevisiae and selenium-enriched yeast. Both treatments were able to reduce myeloperoxidase activity, but only selenium-enriched yeast reduced eosinophil peroxidase activity. CXCL1/KC levels, histopathological tissue damage and oxidative stress (lipid peroxidation and nitrite production) in the small intestine were reduced by both treatments; however, this reduction was always higher when treatment with selenium-enriched yeast was evaluated. CONCLUSIONS: Results of the present study showed that the oral administration of S. cerevisiae UFMG A-905 protected mice against mucositis induced by 5-FU, and that this effect was potentiated when the yeast was enriched with selenium.
PURPOSE:Gastrointestinal mucositis is a major problem associated with cancer therapy. To minimize these deleterious effects, simultaneous administration of antioxidant components, such as selenium, can be considered. There is a growing interest in the use of yeasts because they are able to convert inorganic selenium into selenomethionine. In the present study, oral administration of Saccharomyces cerevisiae UFMG A-905 enriched with selenium was evaluated as an alternative in minimizing the side effects of 5FU-induced mucositis in mice. METHODS:Mice body weight, food consumption, faeces consistency and the presence of blood in faeces were assessed daily during experimental mucositis induced by 5-fluorouracil (5FU). Blood was used for intestinal permeability determination, and small intestine for oxidative stress, immunological and histopathological examination. RESULTS: The increased intestinal permeability observed with mucositis induction was partially reverted by S. cerevisiae and selenium-enriched yeast. Both treatments were able to reduce myeloperoxidase activity, but only selenium-enriched yeast reduced eosinophil peroxidase activity. CXCL1/KC levels, histopathological tissue damage and oxidative stress (lipid peroxidation and nitrite production) in the small intestine were reduced by both treatments; however, this reduction was always higher when treatment with selenium-enriched yeast was evaluated. CONCLUSIONS: Results of the present study showed that the oral administration of S. cerevisiae UFMG A-905 protected mice against mucositis induced by 5-FU, and that this effect was potentiated when the yeast was enriched with selenium.
Authors: Giovanna A Belo; Bárbara F Cordeiro; Emiliano R Oliveira; Marina P Braga; Sara H da Silva; Bruno G Costa; Flaviano Dos S Martins; Gwénaël Jan; Yves Le Loir; Alfonso Gala-García; Enio Ferreira; Vasco Azevedo; Fillipe L R do Carmo Journal: Front Pharmacol Date: 2021-12-20 Impact factor: 5.810
Authors: Joana O P A Coutinho; Taynara S Peixoto; Graciéle C A de Menezes; Camila R Carvalho; Mayara B Ogaki; Eldon C Q Gomes; Carlos A Rosa; Luiz H Rosa; Rosa M E Arantes; Jacques R Nicoli; Fabiana C P Tiago; Flaviano S Martins Journal: Probiotics Antimicrob Proteins Date: 2021-03-23 Impact factor: 4.609
Authors: Thais Fernanda de Campos Fraga-Silva; Luiza Ayumi Nishiyama Mimura; Larissa Ragozo Cardoso de Oliveira; Juliana Helena Dos Santos Toledo; Patrícia Aparecida Borim; Sofia Fernanda Gonçalvez Zorzella-Pezavento; Diego Peres Alonso; Paulo Eduardo Martins Ribolla; Carlos Alberto Ferreira de Oliveira; Denise Morais da Fonseca; Eduardo J Villablanca; Alexandrina Sartori Journal: Sci Rep Date: 2020-12-17 Impact factor: 4.379
Authors: Joana O P A Coutinho; Mônica F Quintanilha; Marina R A Campos; Enio Ferreira; Graciéle C A de Menezes; Luiz H Rosa; Carlos A Rosa; Katia D Vital; Simone O A Fernandes; Valbert N Cardoso; Jacques R Nicoli; Fabiana C P Tiago; Flaviano S Martins Journal: Probiotics Antimicrob Proteins Date: 2021-07-13 Impact factor: 5.265