Zhenjiang Ding1, Jie Liu2, Junting Wang3, Biying Huang3, Ming Zhong4. 1. Department of Pediatric Dentistry, School of Stomatology, China Medical University, Shenyang, Liaoning, China; Department of Oral Histopathology, School of Stomatology, China Medical University, Shenyang, Liaoning, China. 2. Department of Central Laboratory, China Medical University, Shenyang, Liaoning, China. 3. Department of Oral Histopathology, School of Stomatology, China Medical University, Shenyang, Liaoning, China. 4. Department of Oral Histopathology, School of Stomatology, China Medical University, Shenyang, Liaoning, China. Electronic address: Mzhong@cmu.edu.cn.
Abstract
OBJECTIVES: This study aimed to detect the expression of eukaryotic translation initiation factor 3 subunit a (eIF3a) in ameloblastoma (AB) tissues compared with normal oral mucosa (NOM) tissues and investigate the roles of eIF3a in the immortalized ameloblastoma cell line (AM-1) cell proliferation and apoptosis. STUDY DESIGN: We performed immunohistochemistry to determine the expression of eIF3a in AB tissues (n = 83) and NOM tissues (n = 20). Real time-quantitative polymerase chain reaction and Western blot analyses were conducted with AB tissues (n = 30) and NOM tissues (n = 6). The correlation between eIF3a expression and the clinical/pathologic features of patients with AB is also presented. The functional role of eIF3a in AM-1 cells was assessed with lentiviral vector-mediated shRNA (small hairpin RNA). RESULTS: Our results indicated that eIF3a was significantly upregulated in AB. Additionally, eIF3a knockdown in AM-1 cells significantly inhibited cell proliferation and promoted apoptosis. CONCLUSIONS: These data indicate that eIF3a facilitates the survival of AB cells and may serve as a promising therapeutic target in AB.
OBJECTIVES: This study aimed to detect the expression of eukaryotic translation initiation factor 3 subunit a (eIF3a) in ameloblastoma (AB) tissues compared with normal oral mucosa (NOM) tissues and investigate the roles of eIF3a in the immortalized ameloblastoma cell line (AM-1) cell proliferation and apoptosis. STUDY DESIGN: We performed immunohistochemistry to determine the expression of eIF3a in AB tissues (n = 83) and NOM tissues (n = 20). Real time-quantitative polymerase chain reaction and Western blot analyses were conducted with AB tissues (n = 30) and NOM tissues (n = 6). The correlation between eIF3a expression and the clinical/pathologic features of patients with AB is also presented. The functional role of eIF3a in AM-1 cells was assessed with lentiviral vector-mediated shRNA (small hairpin RNA). RESULTS: Our results indicated that eIF3a was significantly upregulated in AB. Additionally, eIF3a knockdown in AM-1 cells significantly inhibited cell proliferation and promoted apoptosis. CONCLUSIONS: These data indicate that eIF3a facilitates the survival of AB cells and may serve as a promising therapeutic target in AB.