Comparative transcriptome analysis of TLR8 signaling cells revealed the porcine TLR8 specific differentially expressed genes.

TLRs are the first discovered family of pattern recognition receptors (PRRs). They recognize pathogen associated molecular patterns (PAMPs) and initiate protective immune response. TLR8 as the main endolysosomal TLR, has recently regained attention especially for its structure and function. We previously found TLR8 exhibits species-specific activation by TLR7 specific agonist, Imiquimod (R837). Thus, we next initiated the identification of porcine TLR8 (pTLR8) specific downstream differentially expressed genes (DEGs) by parallel transcriptome analysis of porcine TLR8 and human TLR8 (hTLR8) signaling stable NF-κB reporter cells activated by TLR8 agonist Resiquimod (R848). It turned out that the two TLR8 NF-κB reporter cells can recapitulate the species-specific activity of pTLR8 and hTLR8, transcriptome analysis revealed a number of pTLR8 specific DEGs activated by R848, and some of these gene expressions were confirmed in porcine alveolar macrophages (PAMs) but not occurred in human cell types.