Martha S van Ginkel1, Erlin A Haacke2, Hendrika Bootsma3, Suzanne Arends3, Jolien F van Nimwegen3, Gwenny M Verstappen3, Fred K L Spijkervet4, Arjan Vissink4, Bert van der Vegt5, Frans G M Kroese3. 1. Department of Rheumatology and Clinical Immunology, University of Groningen, University Medical Centre Groningen, the Netherlands. m.s.van.ginkel@umcg.nl. 2. Department of Rheumatology and Clinical Immunology, and Department of Pathology and Medical Biology, University of Groningen, University Medical Centre Groningen, the Netherlands. 3. Department of Rheumatology and Clinical Immunology, University of Groningen, University Medical Centre Groningen, the Netherlands. 4. Department of Oral and Maxillofacial Surgery, University of Groningen, University Medical Centre Groningen, the Netherlands. 5. Department of Pathology and Medical Biology, University of Groningen, University Medical Centre Groningen, the Netherlands.
Abstract
OBJECTIVES: Lymphoepithelial lesions (LELs) in salivary glands are associated with primary Sjögren's syndrome (pSS). LELs are composed of hyperplastic epithelium infiltrated with lymphocytes. The objective of this study was obtaining insight in the relative roles of intraepithelial B- and T-lymphocytes in the formation of LELs in salivary glands of pSS patients. METHODS: Parotid and labial salivary gland biopsies of pSS patients (n=15), non-SS sicca patients (n=5) and non-sicca controls (n=5) were analysed. Serial sections were stained with H & E and for cytokeratin, CD20 and CD3. Striated ducts with lymphocytes, but without hyperplasia, and striated ducts with LELs were identified in H & E and cytokeratin stained sections. LELs were classified in successive stages of severity based on the amount of hyperplasia (stage1-3). Numbers of B- and T-lymphocytes within striated ducts and LELs were counted in CD20 and CD3 stained sections. RESULTS: Lymphocyte-containing striated ducts of both salivary glands of all pSS and control patients harboured T-lymphocytes, scattered throughout the ductal epithelium. In contrast, B-lymphocytes were exclusively found in a small fraction (21%) of striated ducts without hyperplasia and in nearly all striated ducts with LELs of pSS patients, but not in controls. In striated ducts with LELs B-lymphocytes were mostly located in the areas of proliferating epithelium. Numbers of B-lymphocytes and B/T-ratios increased significantly with higher severity of LELs. This was even more pronounced in the parotid than in the labial gland. CONCLUSIONS: We conclude there is an association between presence of intraepithelial B-lymphocytes and the formation of LELs in salivary glands of pSS patients.
OBJECTIVES:Lymphoepithelial lesions (LELs) in salivary glands are associated with primary Sjögren's syndrome (pSS). LELs are composed of hyperplastic epithelium infiltrated with lymphocytes. The objective of this study was obtaining insight in the relative roles of intraepithelial B- and T-lymphocytes in the formation of LELs in salivary glands of pSSpatients. METHODS: Parotid and labial salivary gland biopsies of pSSpatients (n=15), non-SS sicca patients (n=5) and non-sicca controls (n=5) were analysed. Serial sections were stained with H & E and for cytokeratin, CD20 and CD3. Striated ducts with lymphocytes, but without hyperplasia, and striated ducts with LELs were identified in H & E and cytokeratin stained sections. LELs were classified in successive stages of severity based on the amount of hyperplasia (stage1-3). Numbers of B- and T-lymphocytes within striated ducts and LELs were counted in CD20 and CD3 stained sections. RESULTS: Lymphocyte-containing striated ducts of both salivary glands of all pSS and control patients harboured T-lymphocytes, scattered throughout the ductal epithelium. In contrast, B-lymphocytes were exclusively found in a small fraction (21%) of striated ducts without hyperplasia and in nearly all striated ducts with LELs of pSSpatients, but not in controls. In striated ducts with LELs B-lymphocytes were mostly located in the areas of proliferating epithelium. Numbers of B-lymphocytes and B/T-ratios increased significantly with higher severity of LELs. This was even more pronounced in the parotid than in the labial gland. CONCLUSIONS: We conclude there is an association between presence of intraepithelial B-lymphocytes and the formation of LELs in salivary glands of pSSpatients.
Authors: Sarah Pringle; Bert van der Vegt; Xiaoyan Wang; Nico van Bakelen; T Jeroen N Hiltermann; Fred K L Spijkervet; Arjan Vissink; Frans G M Kroese; Hendrika Bootsma Journal: Front Oncol Date: 2020-04-02 Impact factor: 6.244
Authors: Gwenny M Verstappen; Lu Gao; Sarah Pringle; Erlin A Haacke; Bert van der Vegt; Silvia C Liefers; Vishal Patel; Yanhua Hu; Sumanta Mukherjee; Julie Carman; Laurence C Menard; Frederik K L Spijkervet; Arjan Vissink; Hendrika Bootsma; Frans G M Kroese Journal: Front Immunol Date: 2021-07-06 Impact factor: 7.561
Authors: Gwenny M Verstappen; John A Ice; Hendrika Bootsma; Sarah Pringle; Erlin A Haacke; Kim de Lange; Gerben B van der Vries; Peter Hickey; Arjan Vissink; Frederik K L Spijkervet; Christopher J Lessard; Frans G M Kroese Journal: J Autoimmun Date: 2020-03-20 Impact factor: 7.094
Authors: Annie Visser; Gwenny M Verstappen; Bert van der Vegt; Arjan Vissink; Richard J Bende; Hendrika Bootsma; Nicolaas A Bos; Frans G M Kroese Journal: Front Immunol Date: 2020-07-14 Impact factor: 7.561
Authors: Xiaoyan Wang; Hendrika Bootsma; Janneke Terpstra; Arjan Vissink; Bert van der Vegt; Fred K L Spijkervet; Frans G M Kroese; Sarah Pringle Journal: Rheumatology (Oxford) Date: 2020-10-01 Impact factor: 7.580