Literature DB >> 3107426

Extraction of starch by dimethyl sulfoxide and quantitation by enzymatic assay.

N C Carpita, J Kanabus.   

Abstract

A method for the rapid, sensitive, and specific determination of starch in plant tissues is described. Starch from a variety of plant tissues is solubilized by stirring for 24 h or by sonication for 40 min in dimethyl sulfoxide. Dilution of this extract to less than 20% dimethyl sulfoxide permits a nearly complete hydrolysis of the starch in less than 3 h with glucoamylase from Rhizopus niveus. Quantitation of liberated glucose by a coupled hexokinase and glucose-6-phosphate dehydrogenase method provides an additional degree of specificity.

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Year:  1987        PMID： 3107426     DOI： 10.1016/0003-2697(87)90662-2

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  14 in total

1.  Overexpression of the carbohydrate binding module of strawberry expansin2 in Arabidopsis thaliana modifies plant growth and cell wall metabolism.

Authors:  Cristina F Nardi; Natalia M Villarreal; Franco R Rossi; Santiago Martínez; Gustavo A Martínez; Pedro M Civello
Journal:  Plant Mol Biol       Date:  2015-04-03       Impact factor: 4.076

2.  Structural analysis of the cell walls regenerated by carrot protoplasts.

Authors:  E M Shea; D M Gibeaut; N C Carpita
Journal:  Planta       Date:  1989-10       Impact factor: 4.116

3.  Cell wall and membrane-associated exo-beta-D-glucanases from developing maize seedlings.

Authors:  J B Kim; A T Olek; N C Carpita
Journal:  Plant Physiol       Date:  2000-06       Impact factor: 8.340

4.  The mechanism of synthesis of a mixed-linkage (1-->3), (1-->4)beta-D-glucan in maize. Evidence for multiple sites of glucosyl transfer in the synthase complex

Authors: 
Journal:  Plant Physiol       Date:  1999-08       Impact factor: 8.340

5.  Phosphate starvation responses are mediated by sugar signaling in Arabidopsis.

Authors:  Athikkattuvalasu S Karthikeyan; Deepa K Varadarajan; Ajay Jain; Michael A Held; Nicholas C Carpita; Kashchandra G Raghothama
Journal:  Planta       Date:  2007-03       Impact factor: 4.116

6.  Chemical structure of the cell walls of dwarf maize and changes mediated by gibberellin.

Authors:  N C Carpita; J Kanabus
Journal:  Plant Physiol       Date:  1988-11       Impact factor: 8.340

7.  Tracing cell wall biogenesis in intact cells and plants : selective turnover and alteration of soluble and cell wall polysaccharides in grasses.

Authors:  D M Gibeaut; N C Carpita
Journal:  Plant Physiol       Date:  1991-10       Impact factor: 8.340

8.  Changes in Esterification of the Uronic Acid Groups of Cell Wall Polysaccharides during Elongation of Maize Coleoptiles.

Authors:  J B Kim; N C Carpita
Journal:  Plant Physiol       Date:  1992-02       Impact factor: 8.340

9.  The MUR3 gene of Arabidopsis encodes a xyloglucan galactosyltransferase that is evolutionarily related to animal exostosins.

Authors:  Michael Madson; Christophe Dunand; Xuemei Li; Rajeev Verma; Gary F Vanzin; Jeffrey Caplan; Douglas A Shoue; Nicholas C Carpita; Wolf-Dieter Reiter
Journal:  Plant Cell       Date:  2003-07       Impact factor: 11.277

10.  Synthesis and Turnover of Cell-Wall Polysaccharides and Starch in Photosynthetic Soybean Suspension Cultures.

Authors:  V. V. Lozovaya; O. A. Zabotina; J. M. Widholm
Journal:  Plant Physiol       Date:  1996-07       Impact factor: 8.340
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