Correction: Multi-Method Approach for Characterizing the Interaction between Fusarium verticillioides and Bacillus thuringiensis Subsp. Kurstaki.

[This corrects the article DOI: 10.1371/journal.pone.0092189.].

Following publication of this article [1] and the posting of Corrections to Figures 2 and 4 [2, 3], concerns were raised about duplicate flow cytometry dot plots in Figs 2 and 3.

Fig 2

Flow cytometry analysis for Bacillus thuringiensis subsp. kurstaki (Btk) interacting with Fusarium verticillioides (Fv).

Histograms show the number of cells versus the fluorescence intensity. Dot plot graphs show the cell size (SSC) versus the cellular complexity and the SSC versus the fluorescence intensity. The vertical lines define the baseline above which the fluorescence is positive. Figure 2(A). Calcofluor White (CFW) controls and their respective histograms: negative, Btk stained with CFW (a.1); negative, Fv cells not stained with CFW (a.2); and positive, fungal cells stained with CFW (a.3). Figure 2(B). 7-Aminoactinomycin (7-AAD) controls: negative, living Btk cells (b.1.); negative, living Fv cells (b.2.); positive, dead Btk cells (b.3.); and positive, dead Fv cells (b.4.). The fungal cells were gated based on the forward (FSC) and side scatter (SSC) and previous analyses with 7AAD. Figure 2(C). Analysis of the Fv and Fv+Btk cells labeled with CFW and 7-AAD after 3 (c.1), 5 (c.2), and 7 (c.3) days.

Fig 3

Flow cytometry analysis for quantification of Cry 1Ab toxins.

Histograms show the number of Cry 1Ab toxins against the fluorescence intensity. Dot plot graphs show protein size (SSC) against fluorescence intensity. The vertical lines define the baseline above which the fluorescence is positive. (A) Experimental controls. (a.1.) Negative control–Btk cell suspension treated with secondary antibody coupled with Alexa Fluor 488; (a.2.) Fv cells treated with the first and secondary antibodies; (a.3.) Positive control–Btk cell suspension treated with antibody to Cry 1Ab toxin and secondary antibody. (B) Analysis of Cry 1Ab production during 3, 5 and 7 days of Fv + Btk interaction. (b.1.) Third day: Btk + Fv interaction and Btk Cry 1Ab toxin stained with Alexa Fluor 488; (b.2.) fifth day; (b.3.) seventh day. * Fifth day, Btk alone was also used as positive control for Btk, with the purpose of showing the positive histogram for Cry 1Ab marked with Alexa Fluor 488.

There is an error in Fig 2 in both the original article and the previous correction [1,2]. Specifically, the b.1.“Btk 7-AAD negative” panel is a duplicate of the b.2. “Fv 7-AAD negative” panel. Here a revised Fig 2 is provided, with the b.1. “Btk 7-AAD negative” panel replaced with the correct plot from the original experiment.

Additionally, in Fig 3, the a.3. “Btk cry 1Ab positive” and b.2. “Fifth day Btk alone” panels are duplicates because they represent the same sample. There is an error in the legend of Fig 3, which incorrectly states that the a.3. panel shows a mixed Btk+Fv cell suspension. The a.3. panel shows day 5 Btk cells alone treated with anti-cry 1Ab toxin and secondary antibodies. A revised Fig 3 is provided with accompanying revised figure legend as follows:

Flow cytometry analysis output files for the above panels are provided as Supporting Information files below. Additionally, the raw .fcs files for the above panels and additional replicates are provided as Supporting Information. However, the raw flow cytometry data for the other panels in Figs 2 and 3 and the underlying data files for the other figures are no longer available.

The previous Correction to Figure 4 [3] continues to apply.

Fig 2 Data.

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Fig 3 Data.

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