Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles.

Literature DB >> 3106977

Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles.

R J Leslie, R B Hird, L Wilson, J R McIntosh, J M Scholey.

Abstract

Sea urchin embryos in second division have been lysed into microtubule-stabilizing buffers to yield mitotic cytoskeletons (MCSs) that consist of two mitotic spindles surrounded by a cortical array of filaments. Microtubules have been completely extracted from MCSs by incubation at 0 degrees C with Ca2+-containing buffer. An antibody to the microtubule translocator kinesin stains the spindles in MCSs and in MCSs treated with 5 mM ATP and also stains spindle-remnants of the MCSs after the microtubules have been extracted. We conclude that kinesin binds to a nonmicrotubule component in the mitotic spindle. Based on these results, we present several models of kinesin function in the spindle.

Entities: Chemical Species

Mesh：

Substances：

Year: 1987 PMID： 3106977 PMCID： PMC304740 DOI： 10.1073/pnas.84.9.2771

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

25 in total

1. The role of membranes in the ogranization of the mitotic apparatus.

Authors: P Harris
Journal: Exp Cell Res Date: 1975-09 Impact factor: 3.905

2. High resolution two-dimensional electrophoresis of basic as well as acidic proteins.

Authors: P Z O'Farrell; H M Goodman; P H O'Farrell
Journal: Cell Date: 1977-12 Impact factor: 41.582

3. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

Review 4. Ultrastructure of the mitotic spindle.

Authors: H Fuge
Journal: Int Rev Cytol Suppl Date: 1977

Review 5. Polarized intracellular particle transport: saltatory movements and cytoplasmic streaming.

Authors: L I Rebhun
Journal: Int Rev Cytol Date: 1972

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

7. Role of phragmoplast filaments in cell-plate formation.

Authors: A Bajer; R D Allen
Journal: J Cell Sci Date: 1966-12 Impact factor: 5.285

8. Structural aspects of saltatory particle movement.

Authors: L I Rebhun
Journal: J Gen Physiol Date: 1967-07 Impact factor: 4.086

9. Cross-sectional structure of the central mitotic spindle of Diatoma vulgare. Evidence for specific interactions between antiparallel microtubules.

Authors: K L McDonald; M K Edwards; J R McIntosh
Journal: J Cell Biol Date: 1979-11 Impact factor: 10.539

10. Calcium-labile mitotic spindles isolated from sea urchin eggs (Lytechinus variegatus).

Authors: E D Salmon; R R Segall
Journal: J Cell Biol Date: 1980-08 Impact factor: 10.539

23 in total

1. The nuclear-mitotic apparatus protein is important in the establishment and maintenance of the bipolar mitotic spindle apparatus.

Authors: C H Yang; M Snyder
Journal: Mol Biol Cell Date: 1992-11 Impact factor: 4.138

2. A monoclonal antibody against kinesin inhibits both anterograde and retrograde fast axonal transport in squid axoplasm.

Authors: S T Brady; K K Pfister; G S Bloom
Journal: Proc Natl Acad Sci U S A Date: 1990-02 Impact factor: 11.205

Review 3. Do nuclear envelope and intranuclear proteins reorganize during mitosis to form an elastic, hydrogel-like spindle matrix?

Authors: Kristen M Johansen; Arthur Forer; Changfu Yao; Jack Girton; Jørgen Johansen
Journal: Chromosome Res Date: 2011-04 Impact factor: 5.239

Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles.

1. The role of membranes in the ogranization of the mitotic apparatus.

2. High resolution two-dimensional electrophoresis of basic as well as acidic proteins.

3. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Review 4. Ultrastructure of the mitotic spindle.

Review 5. Polarized intracellular particle transport: saltatory movements and cytoplasmic streaming.

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

7. Role of phragmoplast filaments in cell-plate formation.

8. Structural aspects of saltatory particle movement.

9. Cross-sectional structure of the central mitotic spindle of Diatoma vulgare. Evidence for specific interactions between antiparallel microtubules.

10. Calcium-labile mitotic spindles isolated from sea urchin eggs (Lytechinus variegatus).

1. The nuclear-mitotic apparatus protein is important in the establishment and maintenance of the bipolar mitotic spindle apparatus.

2. A monoclonal antibody against kinesin inhibits both anterograde and retrograde fast axonal transport in squid axoplasm.

Review 3. Do nuclear envelope and intranuclear proteins reorganize during mitosis to form an elastic, hydrogel-like spindle matrix?

Review 4. A membranous spindle matrix orchestrates cell division.

5. A computational model for the formation of lamin-B mitotic spindle envelope and matrix.

6. Drosophila kinesin: characterization of microtubule motility and ATPase.

7. Phase transition of spindle-associated protein regulate spindle apparatus assembly.

8. New spindle morphogenesis model by Dynein, Nudel, and the spindle matrix.

9. Isolation and characterization of the gene encoding the heavy chain of Drosophila kinesin.

10. Eg5 is static in bipolar spindles relative to tubulin: evidence for a static spindle matrix.