Jian Zhu1, Yan Yang2, Sigan Hu1, Hui Li1, Heng Zhang1. 1. Department of Cardiology, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China. 2. Department of Medical Oncology, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.
Abstract
OBJECTIVE: To investigate the expression of connexin 43 (Cx43) in peripheral blood monocytes (PBMCs) from patients with acute coronary syndrome (ACS) and its clinical implications. METHODS: We prospectively collected the clinical data from 40 patients with ACS including 20 with unstable angina pectoris (UAP) and 20 with acute myocardial infarction (AMI) admitted in our department between January, 2018 and June, 2018, with 20 healthy subjects undergoing routine physical examinations serving as the control group. Peripheral blood samples were obtained from all the participants and plasma and PBMCs were separated. Enzyme-linked immunosorbent assay (ELISA) and turbidimetric inhibition immunoassay (TIIA) were used for analysis of plasma levels of interleukin (IL)-1β and high sensitive C-reactive protein (hs-CRP), respectively; real-time quantitative RT-PCR and Western blotting were used to detect the mRNA and protein levels of Cx43 in the PBMCs. RESULTS: Compared with the control group, the patients with UAP showed significantly increased plasma levels of IL-1β and hs-CRP (P < 0.001) and obviously elevated expressions of Cx43 at both mRNA and protein levels in the PBMCs (P < 0.001). Compared with the patients with UAP, the patients with AMI had significantly higher plasma IL-1β and hs-CRP levels (P < 0.001 and P < 0.01) but lower expression levels of Cx43 in the PBMCs (P < 0.05). CONCLUSIONS: Patients with UAP and AMI have activated inflammatory responses and reverse changes in Cx43 expression in the PBMCs, suggesting the different roles of Cx43 in the pathogenic mechanisms of different types of ACS.
OBJECTIVE: To investigate the expression of connexin 43 (Cx43) in peripheral blood monocytes (PBMCs) from patients with acute coronary syndrome (ACS) and its clinical implications. METHODS: We prospectively collected the clinical data from 40 patients with ACS including 20 with unstable angina pectoris (UAP) and 20 with acute myocardial infarction (AMI) admitted in our department between January, 2018 and June, 2018, with 20 healthy subjects undergoing routine physical examinations serving as the control group. Peripheral blood samples were obtained from all the participants and plasma and PBMCs were separated. Enzyme-linked immunosorbent assay (ELISA) and turbidimetric inhibition immunoassay (TIIA) were used for analysis of plasma levels of interleukin (IL)-1β and high sensitive C-reactive protein (hs-CRP), respectively; real-time quantitative RT-PCR and Western blotting were used to detect the mRNA and protein levels of Cx43 in the PBMCs. RESULTS: Compared with the control group, the patients with UAP showed significantly increased plasma levels of IL-1β and hs-CRP (P &lt; 0.001) and obviously elevated expressions of Cx43 at both mRNA and protein levels in the PBMCs (P &lt; 0.001). Compared with the patients with UAP, the patients with AMI had significantly higher plasma IL-1β and hs-CRP levels (P &lt; 0.001 and P &lt; 0.01) but lower expression levels of Cx43 in the PBMCs (P &lt; 0.05). CONCLUSIONS:Patients with UAP and AMI have activated inflammatory responses and reverse changes in Cx43 expression in the PBMCs, suggesting the different roles of Cx43 in the pathogenic mechanisms of different types of ACS.
Authors: Christoph J Binder; Mi-Kyung Chang; Peter X Shaw; Yury I Miller; Karsten Hartvigsen; Asheesh Dewan; Joseph L Witztum Journal: Nat Med Date: 2002-11 Impact factor: 53.440
Authors: Ph Gabriel Steg; Stefan K James; Dan Atar; Luigi P Badano; Carina Blömstrom-Lundqvist; Michael A Borger; Carlo Di Mario; Kenneth Dickstein; Gregory Ducrocq; Francisco Fernandez-Aviles; Anthony H Gershlick; Pantaleo Giannuzzi; Sigrun Halvorsen; Kurt Huber; Peter Juni; Adnan Kastrati; Juhani Knuuti; Mattie J Lenzen; Kenneth W Mahaffey; Marco Valgimigli; Arnoud van 't Hof; Petr Widimsky; Doron Zahger Journal: Eur Heart J Date: 2012-08-24 Impact factor: 29.983
Authors: Paul M Ridker; Brendan M Everett; Tom Thuren; Jean G MacFadyen; William H Chang; Christie Ballantyne; Francisco Fonseca; Jose Nicolau; Wolfgang Koenig; Stefan D Anker; John J P Kastelein; Jan H Cornel; Prem Pais; Daniel Pella; Jacques Genest; Renata Cifkova; Alberto Lorenzatti; Tamas Forster; Zhanna Kobalava; Luminita Vida-Simiti; Marcus Flather; Hiroaki Shimokawa; Hisao Ogawa; Mikael Dellborg; Paulo R F Rossi; Roland P T Troquay; Peter Libby; Robert J Glynn Journal: N Engl J Med Date: 2017-08-27 Impact factor: 91.245
Authors: Nan Wang; Elke De Vuyst; Raf Ponsaerts; Kerstin Boengler; Nicolás Palacios-Prado; Joris Wauman; Charles P Lai; Marijke De Bock; Elke Decrock; Mélissa Bol; Mathieu Vinken; Vera Rogiers; Jan Tavernier; W Howard Evans; Christian C Naus; Feliksas F Bukauskas; Karin R Sipido; Gerd Heusch; Rainer Schulz; Geert Bultynck; Luc Leybaert Journal: Basic Res Cardiol Date: 2012-11-08 Impact factor: 17.165