Zhiyi Zhou1, Xiaojuan Li1, Guoqing Jiang1, Jue Wang2,3,4, Yuan Qian2,3,4. 1. Department of Obstetrics First Affiliated Hospital of Kunming Medical University,, Kunming 650032, China. 2. Clinical Laboratory, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China. 3. Yunnan Provincial Key Laboratory of Laboratory Medicine, Kunming 650032, China. 4. Yunnan Provincial Institute of Laboratory Diagnosis, Kunming 650032, China.
Abstract
OBJECTIVE: To investigate the effect of vitamin D on microRNA-21(miR-21) expression and migration and invasion of human placental trophoblast cells. METHODS: The changes in the expression of miR-21 were detected using RT-qPCR in HTR-8/SVneo cells following stimulation by vitamin D at different doses for 24, 48 and 72 h.HTR-8/SVneo cells transfected with miR-21 mimic or inhibitor with or without vitamin D treatment were examined for changes in cell migration and invasion abilities using Transwell assay, and Western blotting was used to detect protein expressions of E-cadherin, fibronectin, and MMP9. RESULTS: Vitamin D obviously inhibited the expression of micoRNA-21 in HTR-8/SVneo cells in a concentration-and time-dependent manner.Transfection with the miR-21 mimic significantly inhibited the migration and invasion of HTR-8/SVneo cells, and this inhibitory effect was abolished by treatment with vitamin D; transfection with miR-21 inhibitor obviously promoted the migration and invasion of HTR-8/SVneo cells, and these effects were not significantly affected by vitamin D treatment. CONCLUSIONS: Vitamin D may promote trophoblast cell migration and invasion to accelerate the development of preeclampsia by down-regulating the expression of miR-21.
OBJECTIVE: To investigate the effect of vitamin D on microRNA-21(miR-21) expression and migration and invasion of human placental trophoblast cells. METHODS: The changes in the expression of miR-21 were detected using RT-qPCR in HTR-8/SVneo cells following stimulation by vitamin D at different doses for 24, 48 and 72 h.HTR-8/SVneo cells transfected with miR-21 mimic or inhibitor with or without vitamin D treatment were examined for changes in cell migration and invasion abilities using Transwell assay, and Western blotting was used to detect protein expressions of E-cadherin, fibronectin, and MMP9. RESULTS:Vitamin D obviously inhibited the expression of micoRNA-21 in HTR-8/SVneo cells in a concentration-and time-dependent manner.Transfection with the miR-21 mimic significantly inhibited the migration and invasion of HTR-8/SVneo cells, and this inhibitory effect was abolished by treatment with vitamin D; transfection with miR-21 inhibitor obviously promoted the migration and invasion of HTR-8/SVneo cells, and these effects were not significantly affected by vitamin D treatment. CONCLUSIONS:Vitamin D may promote trophoblast cell migration and invasion to accelerate the development of preeclampsia by down-regulating the expression of miR-21.
Authors: Yiying Wu; Yi Song; Yao Xiong; Xiaodong Wang; Ke Xu; Bin Han; Yang Bai; Li Li; Yuanyuan Zhang; Liming Zhou Journal: Cell Physiol Biochem Date: 2017-09-29
Authors: Lauren A Griggs; Nadiah T Hassan; Roshni S Malik; Brian P Griffin; Brittany A Martinez; Lynne W Elmore; Christopher A Lemmon Journal: Matrix Biol Date: 2017-01-19 Impact factor: 11.583