Ozguc Takmaz1, Dilara Yozgatli2, Selin Ozaltin3, Esra Ozbasli4, Yucel Kocyigit5, Sibel Bulgurcuoglu Kuran6, Huri Bulut7, Mete Gungor4, Faruk Buyru5, Ercan Bastu4. 1. Department of Obstetrics and Gynecology, Acibadem Mehmet Ali Aydinlar University, Buyukdere Cd. No:40, Maslak, 34457, Sariyer/İstanbul, Turkey. ozguctakmaz@hotmail.com. 2. Cerrahpasa School of Medicine, Istanbul University, Istanbul, Turkey. 3. Department of Obstetrics and Gynecology, Acibadem Maslak Hospital, Istanbul, Turkey. 4. Department of Obstetrics and Gynecology, Acibadem Mehmet Ali Aydinlar University, Buyukdere Cd. No:40, Maslak, 34457, Sariyer/İstanbul, Turkey. 5. Department of Obstetrics and Gynecology, Acibadem Fulya Hospital, Istanbul, Turkey. 6. Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Istanbul School of Medicine, Istanbul University, Istanbul, Turkey. 7. Department of Biochemistry, Bezmi Alem University, Istanbul, Turkey.
Abstract
PURPOSE: To evaluate the relationship of clinical pregnancy rates with bone morphogenetic proteins 2-4-7 (BMP 2, 4, 7), growth differentiation factor 9 (GDF 9), and Emmprin levels in follicular fluid of intracytoplasmic sperm injection patients. METHODS: Follicular fluid of 77 patients who underwent ICSI procedure was collected during the oocyte retrieval procedure. And follicular fluid levels of BMP 2, BMP 4, BMP 7, GDF 9, and Emmprin (Basigin) were measured and compared for clinical pregnancy rates. RESULTS: Follicular levels of BMP 4 was significantly higher whereas Emmprin levels were lower in patients who had achieved clinically diagnosed pregnancy compared with those who did not achieve clinical pregnancy after ICSI procedure (P = 0.007 and P = 0.035, respectively). BMP 2, BMP 7, and GDF 9 levels were comparable for both groups. CONCLUSION: Clinical pregnancy rates after ICSI may be associated with follicular fluid levels of Emmprin and BMP 4. Follicular levels of Emmprin and BMP 4 can be used as a marker (as markers for predicting ICSI outcomes) for a better ICSI outcome.
PURPOSE: To evaluate the relationship of clinical pregnancy rates with bone morphogenetic proteins 2-4-7 (BMP 2, 4, 7), growth differentiation factor 9 (GDF 9), and Emmprin levels in follicular fluid of intracytoplasmic sperm injection patients. METHODS: Follicular fluid of 77 patients who underwent ICSI procedure was collected during the oocyte retrieval procedure. And follicular fluid levels of BMP 2, BMP 4, BMP 7, GDF 9, and Emmprin (Basigin) were measured and compared for clinical pregnancy rates. RESULTS: Follicular levels of BMP 4 was significantly higher whereas Emmprin levels were lower in patients who had achieved clinically diagnosed pregnancy compared with those who did not achieve clinical pregnancy after ICSI procedure (P = 0.007 and P = 0.035, respectively). BMP 2, BMP 7, and GDF 9 levels were comparable for both groups. CONCLUSION: Clinical pregnancy rates after ICSI may be associated with follicular fluid levels of Emmprin and BMP 4. Follicular levels of Emmprin and BMP 4 can be used as a marker (as markers for predicting ICSI outcomes) for a better ICSI outcome.