Literature DB >> 3106412

DNA probes for detection and identification of Mycoplasma pneumoniae and Mycoplasma genitalium.

H C Hyman, D Yogev, S Razin.   

Abstract

DNA probes specific for Mycoplasma pneumoniae and Mycoplasma genitalium were selected from genomic libraries prepared in pUC13. The 32P-labeled probes could detect, by dot blot hybridization, down to about 0.1 ng of the specific mycoplasma DNA or 10(5) CFU. Biotinylation of probe decreased the sensitivity of detection and produced nonspecific background reactions with nonhomologous DNAs. Sulfonation of probe yielded a similar level of sensitivity with less background.

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Year:  1987        PMID： 3106412      PMCID： PMC266068          DOI： 10.1128/jcm.25.4.726-728.1987

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  11 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Modification of cytidine residues with a bisulfite-O-methylhydroxylamine mixture.

Authors:  E D Verdlov; G S Monastyrskaya; L I Guskova; T L Levitan; V I Sheichenko; E I Budowsky
Journal:  Biochim Biophys Acta       Date:  1974-03-08

3.  Genome size of mycoplasmal DNA.

Authors:  A L Bak; F T Black; C Christiansen; E A Freundt
Journal:  Nature       Date:  1969-12-20       Impact factor: 49.962

4.  The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

Authors:  J Vieira; J Messing
Journal:  Gene       Date:  1982-10       Impact factor: 3.688

5.  Microbiological and serological study of non-gonococcal urethritis with special reference to Mycoplasma genitalium.

Authors:  D Taylor-Robinson; P M Furr; N F Hanna
Journal:  Genitourin Med       Date:  1985-10

6.  Unexpectedly high frequency of antibody to Mycoplasma pneumoniae in human sera as measured by sensitive techniques.

Authors:  H Brunner; B Prescott; H Greenberg; W D James; R L Horswood; R M Chanock
Journal:  J Infect Dis       Date:  1977-04       Impact factor: 5.226

7.  Enhanced isolation of Mycoplasma pneumoniae from throat washings with a newly-modified culture medium.

Authors:  J G Tully; D L Rose; R F Whitcomb; R P Wenzel
Journal:  J Infect Dis       Date:  1979-04       Impact factor: 5.226

8.  Antigenic determinants of the attachment protein of Mycoplasma pneumoniae shared by other pathogenic Mycoplasma species.

Authors:  W A Clyde; P C Hu
Journal:  Infect Immun       Date:  1986-02       Impact factor: 3.441

9.  Serological cross-reactions between Mycoplasma genitalium and Mycoplasma pneumoniae.

Authors:  K Lind; B O Lindhardt; H J Schütten; J Blom; C Christiansen
Journal:  J Clin Microbiol       Date:  1984-12       Impact factor: 5.948

10.  Immunoblotting for determination of the antigenic specificities of antibodies to the Mycoplasmatales.

Authors:  G E Kenny; F D Cartwright
Journal:  Isr J Med Sci       Date:  1984-10
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  21 in total

Review 1.  Molecular diagnosis of Mycoplasma pneumoniae respiratory tract infections.

Authors:  K Loens; D Ursi; H Goossens; M Ieven
Journal:  J Clin Microbiol       Date:  2003-11       Impact factor: 5.948

2.  In vitro susceptibilities of Mycoplasma genitalium to antibiotics.

Authors:  H Renaudin; J G Tully; C Bebear
Journal:  Antimicrob Agents Chemother       Date:  1992-04       Impact factor: 5.191

Review 3.  Molecular biology and respiratory disease. 6. Modern molecular biology and respiratory bacterial infections: a revolution on the horizon.

Authors:  T F Murphy
Journal:  Thorax       Date:  1990-07       Impact factor: 9.139

4.  The use of subtractive hybridization to obtain a DNA probe specific for Pseudomonas solanacearum race 3.

Authors:  D Cook; L Sequeira
Journal:  Mol Gen Genet       Date:  1991-07

5.  Construction and Use of a Nonradioactive DNA Hybridization Probe for Detection of Pseudomonas syringae pv. Tomato on Tomato Plants.

Authors:  D A Cuppels; R A Moore; V L Morris
Journal:  Appl Environ Microbiol       Date:  1990-06       Impact factor: 4.792

6.  Identification and classification of Campylobacter strains by using nonradioactive DNA probes.

Authors:  D Chevrier; D Larzul; F Megraud; J L Guesdon
Journal:  J Clin Microbiol       Date:  1989-02       Impact factor: 5.948

7.  Immunocytochemical detection of sulphonylated DNA in tissue sections. An alternative to Feulgen staining of DNA.

Authors:  P Fritz; B Oeffinger; H Multhaupt; A Mischlinski; C Klein; E Roller; H V Tuczek
Journal:  Histochemistry       Date:  1989

8.  Comparison of polymerase chain reaction assay with culture for detection of genital mycoplasmas in perinatal infections.

Authors:  N Luki; P Lebel; M Boucher; B Doray; J Turgeon; R Brousseau
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1998-04       Impact factor: 3.267

9.  Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification.

Authors:  F J van Kuppeveld; J T van der Logt; A F Angulo; M J van Zoest; W G Quint; H G Niesters; J M Galama; W J Melchers
Journal:  Appl Environ Microbiol       Date:  1992-08       Impact factor: 4.792

10.  Detection of Mycoplasma pneumoniae in clinical samples from pediatric patients by polymerase chain reaction.

Authors:  L Skakni; A Sardet; J Just; J Landman-Parker; J Costil; N Moniot-Ville; F Bricout; A Garbarg-Chenon
Journal:  J Clin Microbiol       Date:  1992-10       Impact factor: 5.948
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