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Literature DB >> 3106023

Fluorescence correlation spectroscopy in the nanosecond time range: rotational diffusion of bovine carbonic anhydrase B.

P Kask, P Piksarv, U Mets, M Pooga, E Lippmaa.

Abstract

A fluorescence correlation experiment for measurement of rotational diffusion in the nanosecond time scale is described. Using this method, the rotational diffusion coefficient of bovine carbonic anhydrase B labelled with tetramethylrhodamine isothiocyanate was estimated to be Dr = (1.14 +/- 0.15) X 10(7) s-1 at 22 degrees C. The experiment is based on a cw argon ion laser, a microfluorometer with local solution flow inside the sample cell, and two photon detectors. The fluorescence intensity autocorrelation function in the nanosecond time range is computed with the help of a time-to-amplitude converter and a multichannel pulse-amplitude analyser.

Entities: Chemical Species

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Year: 1987 PMID： 3106023 DOI： 10.1007/bf00256359

Source DB: PubMed Journal: Eur Biophys J ISSN： 0175-7571 Impact factor: 1.733

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1. Fluorescence correlation spectroscopy. II. An experimental realization.

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2. Segmental flexibility in an antibody molecule.

Authors: J Yguerabide; H F Epstein; L Stryer
Journal: J Mol Biol Date: 1970-08 Impact factor: 5.469

3. Lateral diffusion in planar lipid bilayers.

Authors: P F Fahey; D E Koppel; L S Barak; D E Wolf; E L Elson; W W Webb
Journal: Science Date: 1977-01-21 Impact factor: 47.728

4. The use of fluorescence correlations spectroscopy to probe chromatin in the cell nucleus.

Authors: S M Sorscher; J C Bartholomew; M P Klein
Journal: Biochim Biophys Acta Date: 1980-11-14

4 in total

11 in total

1. Fluorescence correlation spectroscopy close to a fluctuating membrane.

Authors: Cécile Fradin; Asmahan Abu-Arish; Rony Granek; Michael Elbaum
Journal: Biophys J Date: 2003-03 Impact factor: 4.033

2. Precise boundary element computation of protein transport properties: Diffusion tensors, specific volume, and hydration.

Authors: Sergio Aragon; David K Hahn
Journal: Biophys J Date: 2006-05-19 Impact factor: 4.033

3. Fluorescence correlation spectroscopy simulations of photophysical phenomena and molecular interactions: a molecular dynamics/monte carlo approach.

Authors: James A Dix; Erik F Y Hom; A S Verkman
Journal: J Phys Chem B Date: 2006-02-02 Impact factor: 2.991

4. Separation of the rotational contribution in fluorescence correlation experiments.

Authors: P Kask; P Piksarv; M Pooga; U Mets; E Lippmaa
Journal: Biophys J Date: 1989-02 Impact factor: 4.033

5. Characterizing diffusion dynamics of a membrane protein associated with nanolipoproteins using fluorescence correlation spectroscopy.

Authors: Tingjuan Gao; Craig D Blanchette; Wei He; Feliza Bourguet; Sonny Ly; Federico Katzen; Wieslaw A Kudlicki; Paul T Henderson; Ted A Laurence; Thomas Huser; Matthew A Coleman
Journal: Protein Sci Date: 2011-02 Impact factor: 6.725

Fluorescence correlation spectroscopy in the nanosecond time range: rotational diffusion of bovine carbonic anhydrase B.

1. Fluorescence correlation spectroscopy. II. An experimental realization.

2. Segmental flexibility in an antibody molecule.

3. Lateral diffusion in planar lipid bilayers.

4. The use of fluorescence correlations spectroscopy to probe chromatin in the cell nucleus.

1. Fluorescence correlation spectroscopy close to a fluctuating membrane.

2. Precise boundary element computation of protein transport properties: Diffusion tensors, specific volume, and hydration.

3. Fluorescence correlation spectroscopy simulations of photophysical phenomena and molecular interactions: a molecular dynamics/monte carlo approach.

4. Separation of the rotational contribution in fluorescence correlation experiments.

5. Characterizing diffusion dynamics of a membrane protein associated with nanolipoproteins using fluorescence correlation spectroscopy.

Review 6. Fluorescence correlation spectroscopy: diagnostics for sparse molecules.

7. Fluorescence correlation analysis of probe diffusion simplifies quantitative pathogen detection by PCR.

8. Analysis of quantum rod diffusion by polarized fluorescence correlation spectroscopy.

9. Tritium NMR studies of the human carbonic anhydrase I-benzenesulfonamide complex.

10. Continuous Fluorescence Depletion Anisotropy Measurement of Protein Rotation.