Lei Ding1, Yihang Gong1, Zhengfei Yang2, Baojia Zou1, Xialei Liu1, Baimeng Zhang1, Jian Li3. 1. Department of Hepatobiliary Surgery, The 5th Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong Province, China. 2. Emergency Department, Sun Yat-sen Memorial Hospital, Guangzhou, Guangdong Province, China. 3. Department of Hepatobiliary Surgery, The 5th Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong Province, China. Lijian5@mail.sysu.edu.cn.
Abstract
BACKGROUND: Probiotic use to prevent gastrointestinal infections in critical care has shown great promise in recent clinical trials. Although well-documented benefits of probiotic use in intestinal disorders, the potential for probiotic treatment to ameliorate liver injury and hypoxic hepatitis following sepsis has not been well explored. METHODS: In order to evaluate, if Lactobacillus rhamnosus GG (LGG) treatment in septic rats will protect against liver injury, this study used 20-22-week-old Sprague-Dawley rats which were subjected to cecal ligation and puncture to establish sepsis model and examine mRNA and protein levels of IL-1β, NLRP3, IL-6, TNF-a, VEGF, MCP1, NF-kB and HIF-1α in the liver via real-time PCR, Elisa and Western blot. RESULTS: This study showed that LGG treatment significantly ameliorated liver injury following experimental infection and sepsis. Liver mRNA and protein levels of IL-1β, NLRP3, IL-6, TNF-a, VEGF, MCP1, NF-kB and HIF-1α were significantly reduced in rats receiving LGG. CONCLUSIONS: Thus, our study demonstrated that LGG treatment can reduce liver injury following experimental infection and sepsis and is associated with improved hypoxic hepatitis. Probiotic therapy may be a promising intervention to ameliorate clinical liver injury and hypoxic hepatitis following systemic infection and sepsis.
BACKGROUND: Probiotic use to prevent gastrointestinal infections in critical care has shown great promise in recent clinical trials. Although well-documented benefits of probiotic use in intestinal disorders, the potential for probiotic treatment to ameliorate liver injury and hypoxic hepatitis following sepsis has not been well explored. METHODS: In order to evaluate, if Lactobacillus rhamnosus GG (LGG) treatment in septic rats will protect against liver injury, this study used 20-22-week-old Sprague-Dawley rats which were subjected to cecal ligation and puncture to establish sepsis model and examine mRNA and protein levels of IL-1β, NLRP3, IL-6, TNF-a, VEGF, MCP1, NF-kB and HIF-1α in the liver via real-time PCR, Elisa and Western blot. RESULTS: This study showed that LGG treatment significantly ameliorated liver injury following experimental infection and sepsis. Liver mRNA and protein levels of IL-1β, NLRP3, IL-6, TNF-a, VEGF, MCP1, NF-kB and HIF-1α were significantly reduced in rats receiving LGG. CONCLUSIONS: Thus, our study demonstrated that LGG treatment can reduce liver injury following experimental infection and sepsis and is associated with improved hypoxic hepatitis. Probiotic therapy may be a promising intervention to ameliorate clinical liver injury and hypoxic hepatitis following systemic infection and sepsis.
Authors: Jean Henrion; Michael Schapira; Raymond Luwaert; Lucien Colin; André Delannoy; Françis R Heller Journal: Medicine (Baltimore) Date: 2003-11 Impact factor: 1.889
Authors: Hatem M Abuohashish; Eman H Zaghloul; Amany S El Sharkawy; Eman M Abbas; Mohammed M Ahmed; Salim S Al-Rejaie Journal: Biomed Res Int Date: 2021-12-06 Impact factor: 3.411