Kalliopi Domvri1, Konstantinos Porpodis2, Georgios Tzimagiorgis3, Fani Chatzopoulou4, Theodoros Kontakiotis5, George Kyriazis6, Despina Papakosta7. 1. Department of Pneumonology, Aristotle University of Thessaloniki, General Hospital G.Papanikolaou, Thessaloniki, Greece. Electronic address: kdomvrid@auth.gr. 2. Department of Pneumonology, Aristotle University of Thessaloniki, General Hospital G.Papanikolaou, Thessaloniki, Greece. Electronic address: kporpodis@yahoo.com. 3. Laboratory of Biological Chemistry, School of Health Sciences, Medical Department, Aristotle University of Thessaloniki, Greece. Electronic address: tzimagio@med.auth.gr. 4. Laboratory of Biological Chemistry, School of Health Sciences, Medical Department, Aristotle University of Thessaloniki, Greece. Electronic address: fchatzop@auth.gr. 5. Department of Pneumonology, Aristotle University of Thessaloniki, General Hospital G.Papanikolaou, Thessaloniki, Greece. Electronic address: kontak@auth.gr. 6. Department of Pneumonology, Aristotle University of Thessaloniki, General Hospital G.Papanikolaou, Thessaloniki, Greece. Electronic address: kyriazis@med.auth.gr. 7. Department of Pneumonology, Aristotle University of Thessaloniki, General Hospital G.Papanikolaou, Thessaloniki, Greece. Electronic address: dpapakos@med.auth.gr.
Abstract
OBJECTIVE: The aim of the present study was to investigate the Th2/Th17 pathway in asthmatic patients and also the relationship to asthma severity and biomarkers of inflammation. METHODS: 90 asthmatic patients, 51 patients with severe, 39 patients with mild asthma and 98 healthy controls were included. Skin prick tests, blood eosinophils, total serum IgE and exhaled FeNO were evaluated. Serum levels of IL-4, IL-5, IL-13, IL-6, IL-17A, IL-23 and TGFβ1 were determined by Flow Cytometry using a panel kit (AimPlex Biosciences). The SNP of IL17A (rs17880588) was genotyped using reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: The genotype of the SNP in IL17A (rs 17880588) was similar among all three groups. Serum levels of IL-4, IL-5, IL-13, IL-6, IL-17A and IL-23 were higher in asthmatics compared to controls (p < 0.05). In addition, IL-17A and IL-4 serum levels were found significantly elevated in patients with allergic asthma (p < 0.05). Furthermore, IL-4, IL-5, IL-13 and IL-23 were found significantly higher in patients with eosinophil cut off values above 300 cells/μl (p < 0.05). IL-17A levels were positively correlated with FeNO values in severe asthmatics with eosinophils>400 cells/μl. CONCLUSIONS: The above findings suggest the coexistence of Th2/Th17 pathway in severe, eosinophilic and in allergic asthma.
OBJECTIVE: The aim of the present study was to investigate the Th2/Th17 pathway in asthmatic patients and also the relationship to asthma severity and biomarkers of inflammation. METHODS: 90 asthmatic patients, 51 patients with severe, 39 patients with mild asthma and 98 healthy controls were included. Skin prick tests, blood eosinophils, total serum IgE and exhaled FeNO were evaluated. Serum levels of IL-4, IL-5, IL-13, IL-6, IL-17A, IL-23 and TGFβ1 were determined by Flow Cytometry using a panel kit (AimPlex Biosciences). The SNP of IL17A (rs17880588) was genotyped using reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: The genotype of the SNP in IL17A (rs 17880588) was similar among all three groups. Serum levels of IL-4, IL-5, IL-13, IL-6, IL-17A and IL-23 were higher in asthmatics compared to controls (p < 0.05). In addition, IL-17A and IL-4 serum levels were found significantly elevated in patients with allergic asthma (p < 0.05). Furthermore, IL-4, IL-5, IL-13 and IL-23 were found significantly higher in patients with eosinophil cut off values above 300 cells/μl (p < 0.05). IL-17A levels were positively correlated with FeNO values in severe asthmatics with eosinophils>400 cells/μl. CONCLUSIONS: The above findings suggest the coexistence of Th2/Th17 pathway in severe, eosinophilic and in allergic asthma.
Authors: Juliana de Melo Batista Dos Santos; Roberta Foster; Anne-Charlotte Jonckheere; Marcelo Rossi; Luiz Antonio Luna Junior; Catherine Machado Katekaru; Matheus Cavalcante de Sá; Lucas Guimarães Pagani; Francine Maria de Almeida; Jônatas do Bussador Amaral; Rodolfo de Paula Vieira; Andre Luis Lacerda Bachi; Dominique Magdalena A Bullens; Mauro Vaisberg Journal: Int J Environ Res Public Health Date: 2019-11-12 Impact factor: 3.390
Authors: Ming Jing Wang; Yan Sun; Ying Song; Ju Ning Ma; Zi Qing Wang; Xiao Qing Ding; Hai Yan Chen; Xue Bin Zhang; Min Min Song; Xiao Mei Hu Journal: Front Med (Lausanne) Date: 2022-07-13