Reference-Free Traction Force Microscopy Platform Fabricated via Two-Photon Laser Scanning Lithography Enables Facile Measurement of Cell-Generated Forces.

Cells sense and respond to the physical nature of their microenvironment by mechanically probing their surroundings via cytoskeletal contractions. The material response to these stresses can be measured via traction force microscopy (TFM). Traditional TFM platforms present several limitations including variable spatial resolution, difficulty in attaining the full three-dimensional (3D) deformation/stress profile, and the requirement to remove or relax the cells being measured to determine the zero-stress state. To overcome these limitations, we developed a two-photon, photochemical coupling approach to fabricate a new TFM platform that provides high-resolution control over the 3D placement of fluorescent fiducial markers for facile measurement of cell-generated shear and normal components of traction forces. The highly controlled placement of the 3D marker array provides a built-in, zero stress state eliminating the need to perturb the cells being measured while also providing increased throughput. Using this platform, we discovered that the magnitude of cell-generated shear and normal force components are linked both spatially and temporally. The facile nature and increased throughput of measuring cell-generated forces afforded by this new platform will be useful to the mechanotransduction community and others.