Literature DB >> 31041752

Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Mouse Heart Tissues Using Oroboros 2k-Oxygraph.

Qinqiang Long1, Lizhen Huang2, Kai Huang3, Qinglin Yang4,5.   

Abstract

In this chapter, we describe detailed protocols for measuring high-resolution respirometry on mitochondria extracted from adult whole mouse heart using the Oroboros 2k-Oxygraph system. The method provides detailed procedures for the preparation of mitochondria and measurement of high-resolution respirometry in response to various respiration inhibitions. The method described in this chapter could discern the different respiration rate on mitochondria extracted from two spatially distinct mitochondrial subpopulations, subsarcolemmal mitochondria (SSM) and intermyofibrillar mitochondria (IFM). These approaches can easily be translated to other cells and tissues.

Entities:  

Keywords:  Intermyofibrillar mitochondria (IFM); Mitochondria extraction; Mitochondrial oxygen consumption; Mouse heart; Oxygraph; Subsarcolemmal mitochondria (SSM)

Mesh：

Year:  2019        PMID： 31041752     DOI： 10.1007/978-1-4939-9195-2_19

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  13 in total

1.  The molecular role of Sigmar1 in regulating mitochondrial function through mitochondrial localization in cardiomyocytes.

Authors:  Chowdhury S Abdullah; Richa Aishwarya; Shafiul Alam; Naznin Sultana Remex; Mahboob Morshed; Sadia Nitu; Sumitra Miriyala; Manikandan Panchatcharam; Brandon Hartman; Judy King; Mohammad Alfrad Nobel Bhuiyan; James Traylor; Christopher G Kevil; A Wayne Orr; Md Shenuarin Bhuiyan
Journal:  Mitochondrion       Date:  2021-12-10       Impact factor: 4.160

2.  ATPAF1 deficiency impairs ATP synthase assembly and mitochondrial respiration.

Authors:  Zhou Zhou; Kailiang Zhang; Zhiheng Liu; Xu Gao; Kai Huang; Chen Chen; Daowen Wang; Qinglin Yang; Qinqiang Long
Journal:  Mitochondrion       Date:  2021-08-08       Impact factor: 4.534

3.  EFHD1 ablation inhibits cardiac mitoflash activation and protects cardiomyocytes from ischemia.

Authors:  David R Eberhardt; Sandra H Lee; Xue Yin; Anthony M Balynas; Emma C Rekate; Jackie N Kraiss; Marisa J Lang; Maureen A Walsh; Molly E Streiff; Andrea C Corbin; Ying Li; Katsuhiko Funai; Frank B Sachse; Dipayan Chaudhuri
Journal:  J Mol Cell Cardiol       Date:  2022-03-16       Impact factor: 5.763

Review 4.  Recent Approaches to Determine Static and Dynamic Redox State-Related Parameters.

Authors:  Cristina Mas-Bargues; Esther García-Domínguez; Consuelo Borrás
Journal:  Antioxidants (Basel)       Date:  2022-04-28

5.  Sustained Oligomycin Sensitivity Conferring Protein Expression in Cardiomyocytes Protects Against Cardiac hypertrophy Induced by Pressure Overload via Improving Mitochondrial Function.

Authors:  Yingying Guo; Kailiang Zhang; Xu Gao; Zhou Zhou; Zhiheng Liu; Kevin Yang; Kai Huang; Qinglin Yang; Qinqiang Long
Journal:  Hum Gene Ther       Date:  2020-09-21       Impact factor: 5.695

6.  Mitochondrial respiration in C57BL/6 substrains varies in response to myocardial infarction.

Authors:  Zhou Zhou; Zhiheng Liu; Xu Gao; Qinqiang Long
Journal:  J Bioenerg Biomembr       Date:  2021-02-25       Impact factor: 2.945

7.  Mitochondrial Dysfunction in Cardiorenal Syndrome 3: Renocardiac Effect of Vitamin C.

Authors:  Raquel Silva Neres-Santos; Carolina Victoria Cruz Junho; Karine Panico; Wellington Caio-Silva; Joana Claudio Pieretti; Juliana Almeida Tamashiro; Amedea Barozzi Seabra; César Augusto João Ribeiro; Marcela Sorelli Carneiro-Ramos
Journal:  Cells       Date:  2021-11-05       Impact factor: 6.600

8.  DJ-1 attenuates the glycation of mitochondrial complex I and complex III in the post-ischemic heart.

Authors:  Yvanna Pantner; Rohini Polavarapu; Lih-Shen Chin; Lian Li; Yuuki Shimizu; John W Calvert
Journal:  Sci Rep       Date:  2021-09-30       Impact factor: 4.996

9.  Mitochondrial Toxicity Associated with Imatinib and Sorafenib in Isolated Rat Heart Fibers and the Cardiomyoblast H9c2 Cell Line.

Authors:  Jamal Bouitbir; Miljenko V Panajatovic; Stephan Krähenbühl
Journal:  Int J Mol Sci       Date:  2022-02-18       Impact factor: 5.923

10.  Neuronal induction and bioenergetics characterization of human forearm adipose stem cells from Parkinson's disease patients and healthy controls.

Authors:  Ingrid González-Casacuberta; Dolores Vilas; Claustre Pont-Sunyer; Ester Tobías; Judith Cantó-Santos; Laura Valls-Roca; Francesc Josep García-García; Glòria Garrabou; Josep Maria Grau-Junyent; Maria Josep Martí; Francesc Cardellach; Constanza Morén
Journal:  PLoS One       Date:  2022-03-15       Impact factor: 3.240
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