Literature DB >> 31041741

Immunofluorescence Labeling of Nuclear Receptor Expression in Formalin-Fixed, Paraffin-Embedded Tissue.

Elizabeth D Lightbody1,2, Christopher J B Nicol3,4,5.   

Abstract

Immunofluorescent staining (IF) uses antigen-antibody complexes tagged with fluorochromes to observe the expression of proteins within a tissue sample. Multiple groups have described optimized methods to visualize several proteins simultaneously within the same tissue section using immunofluorescence in both mouse and human FFPE tissues. Our group routinely uses an optimized protocol described here to examine nuclear receptor expression in experimental samples from conditional knockout in vivo studies.

Entities:  

Keywords:  FFPE; Immunofluorescent labeling; Nuclear receptor

Mesh：

Substances：

Year:  2019        PMID： 31041741     DOI： 10.1007/978-1-4939-9195-2_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

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Journal:  Am J Transl Res       Date:  2021-01-15       Impact factor: 4.060

2.  In Situ Observation of Abscisic Acid Distribution in Major Crop Species by Immunofluorescence Labeling.

Authors:  Mo-Xian Chen; Chong-Chong Lu; Jianhua Zhang; Ying-Gao Liu
Journal:  Methods Mol Biol       Date:  2022

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Journal:  Front Pharmacol       Date:  2021-11-29       Impact factor: 5.810
  3 in total

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