| Literature DB >> 31036320 |
Tao Shu1, Lei He1, Xuan Wang1, Mao Pang1, Bu Yang1, Feng Feng1, Zizhao Wu1, Chang Liu1, Shufan Zhang2, Bin Liu1, Qiyou Wang3, Limin Rong4.
Abstract
Long noncoding RNA (lncRNAs) UCA1 has been known to be critical for the chondrogenic differentiation of marrow mesenchymal stem cells (MSCs). In this study, we explore the effects and mechanisms of UCA1 on the promotion of chondrogenesis of MSCs. During the processes of chondrogenic differentiation of MSCs, UCA1, miRNA-145-5p or miRNA-124-3p was overexpressed into MSCs. UCA1 substantially improved chondrogenesis of MSCs. Furthermore, UCA1 obviously down-regulated the expression of miRNA-145-5p and miRNA-124-3p, which attenuated the chondrogenic differentiation of MSCs. In addition, UCA1 significantly stimulated TGF-β pathway member SMAD5 and SMAD4, which is targeted by miRNA-145-5p and miRNA-124-3p. Collectively, these outcomes suggest that UCA1 enhances chondrogenic differentiation of MSCs via the miRNA-145-5p/SMAD5 and miRNA-124-3p/SMAD4 axis.Entities:
Keywords: Chondrogenesis; Mesenchymal stem cell; SMAD; UCA1; miRNA-124-3p; miRNA-145-5p
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Year: 2019 PMID： 31036320 DOI： 10.1016/j.bbrc.2019.04.140
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575