Literature DB >> 310363

The use of cycloheximide-treated cells for isolating trachoma agents under field conditions.

J Schachter, C R Dawson, I Hoshiwara, T Daghfous, J Banks.   

Abstract

Standard procedures for the isolation of Chlamydia trachomatis require pretreatment of the tissue culture cells. We evaluated the use of cycloheximide, an antimetabolite that can be added to the cells with the inoculum. Cycloheximide-treated cells provided a sensitive system for isolating trachoma agents. This system was applicable to field studies as requirements for equipment were minimal and the cells were 2 weeks old when inoculated.

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Year:  1978        PMID： 310363      PMCID： PMC2395658     

Source DB:  PubMed          Journal:  Bull World Health Organ        ISSN： 0042-9686            Impact factor:   9.408


  10 in total

1.  ISOLATION OF THE TRACHOMA AGENT IN CELL CULTURE.

Authors:  F B GORDON; A L QUAN
Journal:  Proc Soc Exp Biol Med       Date:  1965-02

2.  Are chlamydial infections the most prevalent venereal disease?

Authors:  J Schachter; L Hanna; E C Hill; S Massad; C W Sheppard; J E Conte; S N Cohen; K F Meyer
Journal:  JAMA       Date:  1975-03-24       Impact factor: 56.272

3.  Severe endemic trachoma in tunisia. II. A controlled therapy trial of topically applied chlortetracycline and erythromycin.

Authors:  C R Dawson; T Daghfous; M Messadi; I Hoshiwara; D W Vastine; C Yoneda; J Schacter
Journal:  Arch Ophthalmol       Date:  1974-09

4.  Chlamydial infection. Advances in the diagnostic isolation of Chlamydia, including TRIC agent, from the eye, genital tract, and rectum.

Authors:  S Darougar; B R Jones; J R Kinnison; J D Vaughan-Jackson; E M Dunlop
Journal:  Br J Vener Dis       Date:  1972-12

Review 5.  New knowledge of chlamydiae and the diseases they cause.

Authors:  J T Grayston; S Wang
Journal:  J Infect Dis       Date:  1975-07       Impact factor: 5.226

6.  Chlamydiae as agents of sexually transmitted diseases.

Authors:  J Schachter; G Causse; M L Tarizzo
Journal:  Bull World Health Organ       Date:  1976       Impact factor: 9.408

7.  Effect of infection with the meningopneumonitis agent on deoxyribonucleic acid and protein synthesis by its L-cell host.

Authors:  J J Alexander
Journal:  J Bacteriol       Date:  1969-02       Impact factor: 3.490

8.  Severe endemic trachoma in Tunisia.

Authors:  C R Dawson; T Daghfous; M Messadi; I Hoshiwara; J Schachter
Journal:  Br J Ophthalmol       Date:  1976-04       Impact factor: 4.638

9.  Cytology as a guide to the presence of chlamydial inclusions in Giemsa-stained conjunctival smears in severe endemic trachoma.

Authors:  C Yoneda; C R Dawson; T Daghfous; I Hoshiwara; P Jones; M Messadi; J Schachter
Journal:  Br J Ophthalmol       Date:  1975-03       Impact factor: 4.638

10.  Isolation of Chlamydia trachomatis by use of 5-iodo-2-deoxyuridine-treated cells.

Authors:  B B Wentworth; E R Alexander
Journal:  Appl Microbiol       Date:  1974-05
  10 in total
  3 in total

1.  [Occurrence and diagnosis of Chlamydia trachomatis infections in newborns and young infants (author's transl)].

Authors:  C Simon; P Höger; W Toeller; D Kiosz; B Schröder; H D Brackebusch
Journal:  Infection       Date:  1982       Impact factor: 3.553

2.  Use of a Chlamydia trachomatis DNA probe for detection of ocular chlamydiae.

Authors:  D Dean; L Palmer; C R Pant; P Courtright; S Falkow; P O'Hanley
Journal:  J Clin Microbiol       Date:  1989-05       Impact factor: 5.948

3.  The detection of Chlamydia trachomatis by direct immunofluorescence in conjunctival smears from patients with trachoma and patients with ophthalmia neonatorum using a conjugated monoclonal antibody.

Authors:  D C Mabey; S Booth-Mason
Journal:  J Hyg (Lond)       Date:  1986-02
  3 in total

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