Nozha Mayouf1, Noureddine Charef1, Soulef Saoudi2, Abderrahmane Baghiani1, Seddik Khennouf2, Lekhmici Arrar3. 1. Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria. 2. Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria. 3. Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria. Electronic address: lekharrar@hotmail.com.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Asphodelus microcarpus is an important medicinal plant belonging to family Liliaceae. This plant is used in traditional medicine to treat abscesses by local application of the powder; the roots are used against white spots, and specifically used for ear pain. AIM OF THE STUDY: The aim of the present study was to assess the in vitro antioxidant effects and the in vivo anti-inflammatory activity of the different parts methanolic extracts of the Asphodelus microcarpus: aerial part (APME), leaf (LME), stem flowers (SFME) and root (RME). MATERIAL AND METHODS: The antioxidant potency of extracts was evaluated by DPPH (2, 2-diphenyl-l-picrylhydrazyl), ABTS, β-carotene bleaching assays, iron chelating, free hydroxyl radicals (HO•) assays, and reducing power. The anti-inflammatory effect of the extracts was evaluated using the carrageenan induced rat paw edema and xylene-induced ear edema in mice. RESULTS: The estimation of polyphenols and flavonoids showed that the leave methanolic extract contains a high amount of polyphenols and flavonoids: 755.3 ± 0.036 mg Gallic acid equivalent and 42.2 ± 0.043 mg Rutin equivalent/g of dried material, respectively. Oral administration of the APME and RME extract produced significant (p < 0.05) anti-edematogenic effect with a dose of 500 mg/kg in the carrageenan induced paw edema after 6 h (58.04%, 58.75%, respectively). APME, LME; SFME and RME extracts at 100, 300 and 500 mg/kg, exhibited significant (p < 0.05) inhibition of xylene induced ear edema. CONCLUSION: the present study confirms the use of A. microcarpus in traditional medicine as anti-inflammatory agent.
ETHNOPHARMACOLOGICAL RELEVANCE: Asphodelus microcarpus is an important medicinal plant belonging to family Liliaceae. This plant is used in traditional medicine to treat abscesses by local application of the powder; the roots are used against white spots, and specifically used for ear pain. AIM OF THE STUDY: The aim of the present study was to assess the in vitro antioxidant effects and the in vivo anti-inflammatory activity of the different parts methanolic extracts of the Asphodelus microcarpus: aerial part (APME), leaf (LME), stem flowers (SFME) and root (RME). MATERIAL AND METHODS: The antioxidant potency of extracts was evaluated by DPPH (2, 2-diphenyl-l-picrylhydrazyl), ABTS, β-carotene bleaching assays, iron chelating, free hydroxyl radicals (HO•) assays, and reducing power. The anti-inflammatory effect of the extracts was evaluated using the carrageenan induced ratpaw edema and xylene-induced ear edema in mice. RESULTS: The estimation of polyphenols and flavonoids showed that the leave methanolic extract contains a high amount of polyphenols and flavonoids: 755.3 ± 0.036 mg Gallic acid equivalent and 42.2 ± 0.043 mg Rutin equivalent/g of dried material, respectively. Oral administration of the APME and RME extract produced significant (p < 0.05) anti-edematogenic effect with a dose of 500 mg/kg in the carrageenan induced paw edema after 6 h (58.04%, 58.75%, respectively). APME, LME; SFME and RME extracts at 100, 300 and 500 mg/kg, exhibited significant (p < 0.05) inhibition of xylene induced ear edema. CONCLUSION: the present study confirms the use of A. microcarpus in traditional medicine as anti-inflammatory agent.
Authors: Amira A Abdellatef; Moustafa Fathy; Abd El-Salam I Mohammed; Marwa S Abu Bakr; Amal H Ahmed; Hatem S Abbass; Ahmed H El-Desoky; Hiroyuki Morita; Toshio Nikaido; Yoshihiro Hayakawa Journal: J Nat Med Date: 2021-05-14 Impact factor: 2.343
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