Eun-Ji Ko1,2, Young Lim Oh3, Heung Yeol Kim3, Wan Kyu Eo4, Hongbae Kim5, Ki Hyung Kim6,7, Suk Bong Koh8, Mee Sun Ock1, Yung Hyun Choi9, Ari Kim10, Hyun Ho Choi11,12, Eun Joo Park13, Hee-Jae Cha14. 1. Department of Parasitology and Genetics, Kosin University College of Medicine, Busan, Republic of Korea. 2. Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, Republic of Korea. 3. Department of Obstetrics and Gynecology, Kosin University College of Medicine, Busan, Republic of Korea. 4. Department of Internal Medicine, Kyung Hee University, Seoul, Republic of Korea. 5. Department of Obstetrics and Gynecology, Hallym University College of Medicine, Chuncheon, Republic of Korea. 6. Department of Obstetrics and Gynecology, Pusan National University School of Medicine, Busan, Republic of Korea. 7. Biomedical Research Institute and Pusan Cancer Center, Pusan National University Hospital, Busan, Republic of Korea. 8. Department of Obstetrics and Gynecology, School of Medicine, Catholic University of Daegu, Daegu, Republic of Korea. 9. Department of Biochemistry, College of Oriental Medicine, Dongeui University, Busan, Republic of Korea. 10. Department of Obstetrics and Gynecology, Chicago Medical School, Rosalind Franklin University of Medicine and Science, Vernon Hills, IL, USA. 11. Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. 12. Research Institute of Gastroenterology, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. 13. Department of Obstetrics and Gynecology, Eulji Medical Center, Eulji University, Seoul, Republic of Korea. pej3119@eulji.ac.kr. 14. Department of Parasitology and Genetics, Kosin University College of Medicine, Busan, Republic of Korea. hcha@kosin.ac.kr.
Abstract
BACKGROUND: Constitutive photomorphogenic 1 (COP1) is an E3 ubiquitin ligase that regulates important target proteins for cell growth including p27. The tumor suppressor p27 negatively regulates the cell cycle by inhibiting cyclin-dependent kinase. COP1 negatively regulates p27 stability by mediating its nuclear export and degradation. OBJECTIVE: Even if COP1 and p27 are tightly related and have significant roles in tumor progression, the expression patterns and relationship of both proteins in cancer have not yet been studied. METHOD: We analyzed the expression patterns and relationship between COP1 and p27 using an ovarian cancer tissue microarray by dual immunofluorescence analysis. RESULTS: The expression levels of COP1 and p27 proteins were not significantly different between ovarian cancer tissue and normal control tissue. Other clinical data including age, tumor type, tumor grade, and stage were not significantly related to expression of the two proteins. The co-relationship between COP1 and p27 proteins was significantly high (Pearson correlation coefficient 0.79, p = 8.65 × 10-22). CONCLUSIONS: Our results demonstrate that while the expression levels of COP1 and p27 are highly correlated, they are not significantly related to cancer progression in ovarian cancer.
BACKGROUND:Constitutive photomorphogenic 1 (COP1) is an E3 ubiquitin ligase that regulates important target proteins for cell growth including p27. The tumor suppressor p27 negatively regulates the cell cycle by inhibiting cyclin-dependent kinase. COP1 negatively regulates p27 stability by mediating its nuclear export and degradation. OBJECTIVE: Even if COP1 and p27 are tightly related and have significant roles in tumor progression, the expression patterns and relationship of both proteins in cancer have not yet been studied. METHOD: We analyzed the expression patterns and relationship between COP1 and p27 using an ovarian cancer tissue microarray by dual immunofluorescence analysis. RESULTS: The expression levels of COP1 and p27 proteins were not significantly different between ovarian cancer tissue and normal control tissue. Other clinical data including age, tumor type, tumor grade, and stage were not significantly related to expression of the two proteins. The co-relationship between COP1 and p27 proteins was significantly high (Pearson correlation coefficient 0.79, p = 8.65 × 10-22). CONCLUSIONS: Our results demonstrate that while the expression levels of COP1 and p27 are highly correlated, they are not significantly related to cancer progression in ovarian cancer.
Authors: David Dornan; Sheila Bheddah; Kim Newton; William Ince; Gretchen D Frantz; Patrick Dowd; Hartmut Koeppen; Vishva M Dixit; Dorothy M French Journal: Cancer Res Date: 2004-10-15 Impact factor: 12.701
Authors: Anish Bali; Philippa M O'Brien; Lyndal S Edwards; Robert L Sutherland; Neville F Hacker; Susan M Henshall Journal: Clin Cancer Res Date: 2004-08-01 Impact factor: 12.531