Song-Shan Piao1, Bing Shang2. 1. Department of Gastrointestinal Surgery, Affiliated Hospital of Yanbian University, Yanji, Jilin Province, China. 2. Department of Hepatobiliary Surgery, Central Hospital of Tongchuan Mining Bureau, Tongchuan, Shaanxi Province, China 13571586228@139.com.
Abstract
BACKGROUND: Pizotifen, a 5-HT2A receptor antagonist is usually considered as a preventative drug to reduce the frequency of recurrent migraine headaches in previous research. But it is not clear whether Pizotifen exerts therapeutic effect in colon cancer. The objective of this study was to investigate the effect of Pizotifen on the proliferation and migration of colon cancer HCT116 cells. METHODS: We performed cell counting kit-8 (CCK8) assay to evaluate the effects of Pizotifen on HCT116 cells proliferation, and transwell migration and invasion assays to assess cell motility. Then, flow cytometry apoptosis assay was used to evaluate the effect of Pizotifen on cell apoptosis. Finally, western blot assay was used to determine the changes of Wnt signaling pathway related proteins in HCT116 cells after Pizotifen treatment. RESULTS: The results showed that Pizotifen could significantly inhibit HCT116 cells proliferation, migration, and invasion. The results of flow cytometry apoptosis assay demonstrated that Pizotifen could promote the apoptosis of HCT116 cells. The expression of Wnt3a and β-Catenin protein were significantly inhibited, while the expression of E-cadherin was significantly up-regulated in Pizotifen treated HCT116 cells. CONCLUSION: Pizotifen may inhibit HCT116 cells proliferation and migration by suppressing Wnt signaling pathway and it may serve as a potential candidate drug for the treatment of colon cancer in the future.
BACKGROUND:Pizotifen, a 5-HT2A receptor antagonist is usually considered as a preventative drug to reduce the frequency of recurrent migraine headaches in previous research. But it is not clear whether Pizotifen exerts therapeutic effect in colon cancer. The objective of this study was to investigate the effect of Pizotifen on the proliferation and migration of colon cancer HCT116 cells. METHODS: We performed cell counting kit-8 (CCK8) assay to evaluate the effects of Pizotifen on HCT116 cells proliferation, and transwell migration and invasion assays to assess cell motility. Then, flow cytometry apoptosis assay was used to evaluate the effect of Pizotifen on cell apoptosis. Finally, western blot assay was used to determine the changes of Wnt signaling pathway related proteins in HCT116 cells after Pizotifen treatment. RESULTS: The results showed that Pizotifen could significantly inhibit HCT116 cells proliferation, migration, and invasion. The results of flow cytometry apoptosis assay demonstrated that Pizotifen could promote the apoptosis of HCT116 cells. The expression of Wnt3a and β-Catenin protein were significantly inhibited, while the expression of E-cadherin was significantly up-regulated in Pizotifen treated HCT116 cells. CONCLUSION:Pizotifen may inhibit HCT116 cells proliferation and migration by suppressing Wnt signaling pathway and it may serve as a potential candidate drug for the treatment of colon cancer in the future.
Authors: Rafael Moreno-Sánchez; Álvaro Marín-Hernández; Juan C Gallardo-Pérez; Silvia C Pacheco-Velázquez; Diana X Robledo-Cadena; Joaquín Alberto Padilla-Flores; Emma Saavedra; Sara Rodríguez-Enríquez Journal: Front Oncol Date: 2020-04-09 Impact factor: 6.244