Volatile organic compounds of Bacillus atrophaeus HAB-5 inhibit the growth of Colletotrichum gloeosporioides.

The use of fungicides to control plant diseases creates a potential health risk. One alternative to this problem is the biological control, which has been succesfully applied to control plant diseases. Bacillus atrophaeus HAB-5 exhibits a high inhibitory acitivities against different fungal pathogens and suppresses them. The aim of current studies is to produce and identify the antifungal compounds produced by the strain HAB-5. We found that the submerge fermentation harvested from Luria-Bertani (LB) medium had the highest activity against Colletotrichum gloeosporioides. The petroleum ether crude extract was strongly bioactive and its activity was stable after heat treatment, pH treatment, illuminated light as well as ultra violet exposition. The antifungal compounds were purified using gel chromatography column. Based on Gas Chromatography-Mass Spectrometry (GC-MS) analysis, nineteen different volatile organic compounds (VOCs) were identified included the range of alkanes, alkenes, alcohols, and organics acid. Among these identified compounds, Chloroacetic acid, tetradecyl esters followed by Octadecane and Hexadecanoic acid, methyl ester showed antifungal activity against C. gloeosporioides. Our results clearly showed Chloroacetic acid, tetradecyl esters; Octadecane and Hexadecanoic acid, methyl ester are key inhibitory compounds produced by Bacillus atrophaeus HAB-5 against C. gloeosporioides.