Literature DB >> 3102611

A sensitive micro-immunoassay using beta-galactosidase/anti-beta-galactosidase complexes.

H Durbin, W F Bodmer.   

Abstract

This paper describes a new sensitive microELISA based on enzyme/anti-enzyme complexes following an unlabelled antibody bridging step. beta-Galactosidase/anti-beta-galactosidase complexes were made using a monoclonal antibody raised against bacterial (E. coli) beta-galactosidase and enzyme activity was quantified with a fluorogenic substrate. Because of its high sensitivity the assay is particularly suitable for the detection of limited amounts of antigen. One application illustrated is the analysis of Class I and Class II histocompatibility antigens on peripheral blood lymphocytes using 5000 cells/well in 60-well Terasaki or 96-well microtitre plates.

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Year:  1987        PMID： 3102611     DOI： 10.1016/0022-1759(87)90100-1

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  10 in total

1.  New HLA-A2 variants defined by monoclonal antibodies and cytotoxic T lymphocytes.

Authors:  L J Kennedy; L E Wallace; J A Madrigal; A B Rickinson; J G Bodmer
Journal:  Immunogenetics       Date:  1987       Impact factor: 2.846

2.  Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.

Authors:  M J Browning; P Krausa; A Rowan; D C Bicknell; J G Bodmer; W F Bodmer
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-01       Impact factor: 11.205

3.  p53 mutations in colorectal cancer.

Authors:  N R Rodrigues; A Rowan; M E Smith; I B Kerr; W F Bodmer; J V Gannon; D P Lane
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

4.  Intestinal trefoil factor controls the expression of the adenomatous polyposis coli-catenin and the E-cadherin-catenin complexes in human colon carcinoma cells.

Authors:  J A Efstathiou; M Noda; A Rowan; C Dixon; R Chinery; A Jawhari; T Hattori; N A Wright; W F Bodmer; M Pignatelli
Journal:  Proc Natl Acad Sci U S A       Date:  1998-03-17       Impact factor: 11.205

5.  Beta 2-microglobulin gene mutations: a study of established colorectal cell lines and fresh tumors.

Authors:  D C Bicknell; A Rowan; W F Bodmer
Journal:  Proc Natl Acad Sci U S A       Date:  1994-05-24       Impact factor: 11.205

6.  Highly lytic CD8+, alpha beta T-cell receptor cytotoxic T cells with major histocompatibility complex (MHC) class I antigen-directed cytotoxicity in beta 2-microglobulin, MHC class I-deficient mice.

Authors:  S Apasov; M Sitkovsky
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-01       Impact factor: 11.205

7.  Carcinoembryonic antigen functions as an accessory adhesion molecule mediating colon epithelial cell-collagen interactions.

Authors:  M Pignatelli; H Durbin; W F Bodmer
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

8.  An epitope on carcinoembryonic antigen defined by the clinically relevant antibody PR1A3.

Authors:  H Durbin; S Young; L M Stewart; F Wrba; A J Rowan; D Snary; W F Bodmer
Journal:  Proc Natl Acad Sci U S A       Date:  1994-05-10       Impact factor: 11.205

9.  Targeted killing of colorectal cancer cell lines by a humanised IgG1 monoclonal antibody that binds to membrane-bound carcinoembryonic antigen.

Authors:  Pj Conaghan; Sq Ashraf; Mg Tytherleigh; Jl Wilding; E Tchilian; D Bicknell; N Jmcc Mortensen; Wf Bodmer
Journal:  Br J Cancer       Date:  2008-03-18       Impact factor: 7.640

10.  Humanised IgG1 antibody variants targeting membrane-bound carcinoembryonic antigen by antibody-dependent cellular cytotoxicity and phagocytosis.

Authors:  S Q Ashraf; P Umana; E Mössner; T Ntouroupi; P Brünker; C Schmidt; J L Wilding; N J Mortensen; W F Bodmer
Journal:  Br J Cancer       Date:  2009-11-17       Impact factor: 7.640
  10 in total

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