Lu Wang1,2, Chenyang Wang1,2, Ruihu Zhang1,2, Yu Liu3, Chunfang Wang1,2, Guohua Song1,2, Jingjing Yu1,2, Zhaoyang Chen4,5. 1. Laboratory Animal Center of Shanxi Medical University, Shanxi Province, China. 2. Shanxi Key Laboratory of Experimental Animal Science and Animal Model of Human Disease, Shanxi Medical University, Shanxi Province, China. 3. Department of Pharmacology, Shanxi Medical University, Shanxi Province, China. 4. Laboratory Animal Center of Shanxi Medical University, Shanxi Province, China. ccytycn@163.com. 5. Shanxi Key Laboratory of Experimental Animal Science and Animal Model of Human Disease, Shanxi Medical University, Shanxi Province, China. ccytycn@163.com.
Abstract
PURPOSE: Developing animal models for human diseases is critical for studying complex diseases such as type 2 diabetes mellitus (T2DM). Since inbred colonies of Chinese hamsters tend toward spontaneous development of diabetes, we investigated them as a possible model. METHODS: We regarded individuals with fasting blood glucose (FBG) higher than 6.0 mmol/L and post-prandial blood glucose (PBG) higher than 7.0 mmol/L as diabetic based on the mean and 95% frequency distribution values of FBG and PBG. Diabetic hamsters were characterized based on metabolic profiles, histopathological features, and changes in the expression of genes involved in glucose and lipid metabolism. RESULTS: Metabolic analyses showed that diabetic hamsters exhibited mild hyperglycemia, hypertriglyceridemia, glucose intolerance, and insulin resistance. Histopathological analysis revealed that cell nuclei migrated inward in skeletal muscle and obvious partial liver lipid deposition and focal necrosis was found. We additionally observed mild injury, atrophy, and occasional vacuolization in islet cells. Changes in the expression of several genes related to glucose and lipid metabolism were observed. Decreased expression of adiponectin and GLUT4 and increased expression of PPARγ, Akt, and leptin was observed in skeletal muscle. Decreased expression of adiponectin with increased expression of PPARγ and leptin was observed in the liver. CONCLUSIONS: These results indicate that we have established a spontaneous diabetic hamster line that closely mimics human T2DM, which may hold potential for further research on the pathogenesis and treatment of this disease.
PURPOSE: Developing animal models for human diseases is critical for studying complex diseases such as type 2 diabetes mellitus (T2DM). Since inbred colonies of Chinese hamsters tend toward spontaneous development of diabetes, we investigated them as a possible model. METHODS: We regarded individuals with fasting blood glucose (FBG) higher than 6.0 mmol/L and post-prandial blood glucose (PBG) higher than 7.0 mmol/L as diabetic based on the mean and 95% frequency distribution values of FBG and PBG. Diabetic hamsters were characterized based on metabolic profiles, histopathological features, and changes in the expression of genes involved in glucose and lipid metabolism. RESULTS: Metabolic analyses showed that diabetic hamsters exhibited mild hyperglycemia, hypertriglyceridemia, glucose intolerance, and insulin resistance. Histopathological analysis revealed that cell nuclei migrated inward in skeletal muscle and obvious partial liver lipid deposition and focal necrosis was found. We additionally observed mild injury, atrophy, and occasional vacuolization in islet cells. Changes in the expression of several genes related to glucose and lipid metabolism were observed. Decreased expression of adiponectin and GLUT4 and increased expression of PPARγ, Akt, and leptin was observed in skeletal muscle. Decreased expression of adiponectin with increased expression of PPARγ and leptin was observed in the liver. CONCLUSIONS: These results indicate that we have established a spontaneous diabetichamster line that closely mimics human T2DM, which may hold potential for further research on the pathogenesis and treatment of this disease.
Entities:
Keywords:
Chinese hamster; Differentially expressed genes; Glucose intolerance; Insulin resistance; Type 2 diabetes mellitus
Authors: Jung Han Kim; Taryn P Stewart; Morvarid Soltani-Bejnood; Luan Wang; Jennifer M Fortuna; Ola A Mostafa; Naima Moustaid-Moussa; Ahmed M Shoieb; Michael F McEntee; Yun Wang; Lawrence Bechtel; Jürgen K Naggert Journal: J Endocrinol Date: 2006-11 Impact factor: 4.286
Authors: T Yamauchi; J Kamon; Y Minokoshi; Y Ito; H Waki; S Uchida; S Yamashita; M Noda; S Kita; K Ueki; K Eto; Y Akanuma; P Froguel; F Foufelle; P Ferre; D Carling; S Kimura; R Nagai; B B Kahn; T Kadowaki Journal: Nat Med Date: 2002-10-07 Impact factor: 53.440