Assessment of In Ovo Administration of Bifidobacterium bifidum and Bifidobacterium longum on Performance, Ileal Histomorphometry, Blood Hematological, and Biochemical Parameters of Broilers.

Bifidobacterium is one of the most promising probiotics which was recently used as an alternative growth promoter in poultry. This trial was considered to evaluate the potential effects of in ovo administration of Bifidobacterium bifidum and Bifidobacterium longum on growth performance and biochemical parameters of broilers. Three hundred broiler breeder eggs were incubated and candled individually at day 17 of embryogenesis; then, eggs that had live embryos were randomly divided into six groups. The first and second groups were of intact (without injection) and vehicle-injected (sterile distilled water) control groups, respectively. Other groups were in ovo injected with 1 × 109 and 1 × 107 CFU B. bifidum, and 1 × 109 and 1 × 107 CFU B. longum, respectively. Hatchability was significantly (P < 0.001) affected by in ovo inoculation of bifidobacteria strains. Body weight gain and feed conversion ratio were significantly improved (P = 0.014 and 0.019, respectively) in all treatment groups. Protein fractions, aspartate aminotransferase, and alanine aminotransferase activities were not significantly altered by bifidobacteria strains in ovo injection. Regarding ileal histomorphometry parameters, a significant elevation in villus height and villus height/crypt depth ratio values were recorded while crypt depth was insignificantly affected by in ovo treatments. It could be concluded that the tested manual method of in-yolk sac injection at day 17 of incubation is more favorable in easier delivering of bifidobacteria strains which improve broiler growth performance and ileal development without any undesirable effects on blood indices and liver and renal functions.