| Literature DB >> 31023926 |
Chengang Xiang1, Yuanyuan Du1, Gaofan Meng1, Liew Soon Yi1, Shicheng Sun1, Nan Song1, Xiaonan Zhang2, Yiwei Xiao3, Jie Wang3, Zhigang Yi4, Yifang Liu5, Bingqing Xie6, Min Wu2, Jun Shu4, Da Sun6, Jun Jia1, Zhen Liang6, Dong Sun1, Yanxiang Huang7, Yan Shi1, Jun Xu1, Fengmin Lu3, Cheng Li5, Kuanhui Xiang3, Zhenghong Yuan8, Shichun Lu9, Hongkui Deng10,6.
Abstract
The maintenance of terminally differentiated cells, especially hepatocytes, in vitro has proven challenging. Here we demonstrated the long-term in vitro maintenance of primary human hepatocytes (PHHs) by modulating cell signaling pathways with a combination of five chemicals (5C). 5C-cultured PHHs showed global gene expression profiles and hepatocyte-specific functions resembling those of freshly isolated counterparts. Furthermore, these cells efficiently recapitulated the entire course of hepatitis B virus (HBV) infection over 4 weeks with the production of infectious viral particles and formation of HBV covalently closed circular DNA. Our study demonstrates that, with a chemical approach, functional maintenance of PHHs supports long-term HBV infection in vitro, providing an efficient platform for investigating HBV cell biology and antiviral drug screening.Entities:
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Year: 2019 PMID： 31023926 DOI： 10.1126/science.aau7307
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728