Andrii Berbets1, Halyna Koval2, Adrian Barbe1, Olena Albota1, Oleksandr Yuzko1. 1. Department of Obstetrics and Gynecology, Bukovinskij Derzhavnij Medichnij Universitet, Chernivtsi, Ukraine. 2. Department of Clinical Immunology and Endocrinology, Bukovinskij Derzhavnij Medichnij Universitet, Chernivtsi, Ukraine.
Abstract
Purpose: To investigate the levels of melatonin, proinflammatory and anti-inflammatory cytokines in pregnant women with placental insufficiency (PI).Materials and Methods: The PI was manifested as the intrauterine growth restriction syndrome of fetus (IUGR) in the third pregnancy trimester. The control group consisted of 20 women with uncomplicated pregnancy in the same term. The blood concentrations of melatonin, proinflammatory cytokines, such as tumor necrotizing factor-α (TNF-α), interleukin-1-β (IL-1-β), interleukin-6 (IL-6), and anti-inflammatory cytokines, such as interleukin-4 (IL-4), and interleukin-10 (IL-10), were studied. Results: The concentration of melatonin was found to decrease significantly if pregnancy was complicated by intrauterine fetal growth retardation (study group -126.87 ± 14.87 pg/ml, control group -231.25 ± 21.56 pg/ml, p < .001). The levels of proinflammatory cytokines in the study group were significantly higher as compared with the control group (TNF-α: study group -10.05 ± 1.35 pg/ml, control group -5.60 ± 1.50 pg/ml, p < .05; IL-1-β: study group -14.67 ± 2.13 pg/ml, control group -3.96 ± 0.92 pg/ml, p < .001; IL-6: study group -6.91 ± 0.99 pg/ml, control group -2.69 ± 0.99 pg/ml, p < .05). The same is true about anti-inflammatory cytokines (IL-4: study group -5.97 ± 0.50 pg/ml, control group -3.74 ± 0.62 pg/ml, p < .05; IL-10: study group -11.40 ± 1.50 pg/ml, control group -4.70 ± 3.20 pg/ml, p < .001). A moderate negative correlation between melatonin and IL-1-β in the group with PI (r = -0.3776, p = .0097), a closed negative correlation between the same indexes in the control group (r = -0.6785, p = .001), and a moderate negative correlation between melatonin and TNF-α (r = -0.4908, p = .02) were found.Conclusions: The blood level of melatonin significantly decreases in case of placental insufficiency, manifested as intrauterine fetal growth restriction. Strengthening of the proinflammatory immunity shown as the increasing of the levels of TNF-α, IL-1-β, and IL-6 levels is also present in case of IUGR. Increase of the serum concentration of the anti-inflammatory cytokines, such as IL-4 and IL-10, in our opinion, can be explained by activation of compensatory mechanisms, which decrease the risk of premature labor.
Purpose: To investigate the levels of melatonin, proinflammatory and anti-inflammatory cytokines in pregnant women with placental insufficiency (PI).Materials and Methods: The PI was manifested as the intrauterine growth restriction syndrome of fetus (IUGR) in the third pregnancy trimester. The control group consisted of 20 women with uncomplicated pregnancy in the same term. The blood concentrations of melatonin, proinflammatory cytokines, such as tumor necrotizing factor-α (TNF-α), interleukin-1-β (IL-1-β), interleukin-6 (IL-6), and anti-inflammatory cytokines, such as interleukin-4 (IL-4), and interleukin-10 (IL-10), were studied. Results: The concentration of melatonin was found to decrease significantly if pregnancy was complicated by intrauterine fetal growth retardation (study group -126.87 ± 14.87 pg/ml, control group -231.25 ± 21.56 pg/ml, p < .001). The levels of proinflammatory cytokines in the study group were significantly higher as compared with the control group (TNF-α: study group -10.05 ± 1.35 pg/ml, control group -5.60 ± 1.50 pg/ml, p < .05; IL-1-β: study group -14.67 ± 2.13 pg/ml, control group -3.96 ± 0.92 pg/ml, p < .001; IL-6: study group -6.91 ± 0.99 pg/ml, control group -2.69 ± 0.99 pg/ml, p < .05). The same is true about anti-inflammatory cytokines (IL-4: study group -5.97 ± 0.50 pg/ml, control group -3.74 ± 0.62 pg/ml, p < .05; IL-10: study group -11.40 ± 1.50 pg/ml, control group -4.70 ± 3.20 pg/ml, p < .001). A moderate negative correlation between melatonin and IL-1-β in the group with PI (r = -0.3776, p = .0097), a closed negative correlation between the same indexes in the control group (r = -0.6785, p = .001), and a moderate negative correlation between melatonin and TNF-α (r = -0.4908, p = .02) were found.Conclusions: The blood level of melatonin significantly decreases in case of placental insufficiency, manifested as intrauterine fetal growth restriction. Strengthening of the proinflammatory immunity shown as the increasing of the levels of TNF-α, IL-1-β, and IL-6 levels is also present in case of IUGR. Increase of the serum concentration of the anti-inflammatory cytokines, such as IL-4 and IL-10, in our opinion, can be explained by activation of compensatory mechanisms, which decrease the risk of premature labor.
Authors: Dmitry O Ivanov; Inna I Evsyukova; Ekaterina S Mironova; Victoria O Polyakova; Igor M Kvetnoy; Ruslan A Nasyrov Journal: Int J Mol Sci Date: 2021-02-19 Impact factor: 5.923
Authors: Gabriel Mayoral Andrade; Gabriela Vásquez Martínez; Laura Pérez-Campos Mayoral; María Teresa Hernández-Huerta; Edgar Zenteno; Eduardo Pérez-Campos Mayoral; Margarito Martínez Cruz; Ruth Martínez Cruz; Carlos Alberto Matias-Cervantes; Noemi Meraz Cruz; Carlos Romero Díaz; Eli Cruz-Parada; Eduardo Pérez-Campos Journal: Front Immunol Date: 2020-11-19 Impact factor: 7.561
Authors: David Ramiro-Cortijo; María de la Calle; Pilar Rodríguez-Rodríguez; Ángel L López de Pablo; María R López-Giménez; Yolanda Aguilera; María A Martín-Cabrejas; María Del Carmen González; Silvia M Arribas Journal: Antioxidants (Basel) Date: 2020-03-25