Niannian Wang1, Feifei Zhu2, Mingxiang Shen3, Lipeng Qiu4, Min Tang5, Hengchuan Xia6, Liang Chen7, Yi Yuan8, Shangshang Ma9, Keping Chen10. 1. Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China. Electronic address: nnw@ujs.edu.cn. 2. Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China. Electronic address: feifzhu@ujs.edu.cn. 3. Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China. Electronic address: 1185255941@qq.com. 4. Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China. Electronic address: tulip_lipeng@163.com. 5. Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York City, NY, 10029, USA. Electronic address: boymin2010@163.com. 6. Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China. Electronic address: hchxia@ujs.edu.cn. 7. Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China. Electronic address: oochen@ujs.edu.cn. 8. Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China. Electronic address: yuanxiaoyi130704@163.com. 9. Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China. Electronic address: 1326056784@qq.com. 10. Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China. Electronic address: kpchen@ujs.edu.cn.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Potentilla discolor Bunge (PDB) is a commonly used herbal for alleviating diabetes mellitus and its complications. Although accumulating evidences show the anti-diabetic efficacy of PDB, the vital anti-diabetic compounds and their functional targets remain elusive. AIM OF THE STUDY: To investigate the anti-diabetic ingredients and their functional mechanisms in PDB, gas chromatograph-mass spectrometry analysis was performed on PDB extract and 21 were testified as anti-diabetic compounds. MATERIALS AND METHODS: Subsequently their potential protein targets were also identified. The bioinformatics analysis was implemented by network pharmacology-based approaches. STRING analysis was performed to reveal enrichment of these target proteins, protein-protein interactions, pathways and related diseases. Cytoscape was used to determine the potential protein targets for these components in PDB, indicating that 21 anti-diabetic compounds in PDB regulate 33 diabetes-related proteins in 28 signal pathways and involve 21 kinds of diabetes-related diseases. Among the 21 potential anti-diabetic components predicted by network analysis, tricetin was firstly experimentally validated at the molecular and cellular level. RESULTS: Results indicated that this active small-molecule compound may have beneficial effects on improving glucose uptake. CONCLUSIONS: We envisage that network analysis will be useful in screening bioactive compounds of medicinal plants.
ETHNOPHARMACOLOGICAL RELEVANCE: Potentilla discolor Bunge (PDB) is a commonly used herbal for alleviating diabetes mellitus and its complications. Although accumulating evidences show the anti-diabetic efficacy of PDB, the vital anti-diabetic compounds and their functional targets remain elusive. AIM OF THE STUDY: To investigate the anti-diabetic ingredients and their functional mechanisms in PDB, gas chromatograph-mass spectrometry analysis was performed on PDB extract and 21 were testified as anti-diabetic compounds. MATERIALS AND METHODS: Subsequently their potential protein targets were also identified. The bioinformatics analysis was implemented by network pharmacology-based approaches. STRING analysis was performed to reveal enrichment of these target proteins, protein-protein interactions, pathways and related diseases. Cytoscape was used to determine the potential protein targets for these components in PDB, indicating that 21 anti-diabetic compounds in PDB regulate 33 diabetes-related proteins in 28 signal pathways and involve 21 kinds of diabetes-related diseases. Among the 21 potential anti-diabetic components predicted by network analysis, tricetin was firstly experimentally validated at the molecular and cellular level. RESULTS: Results indicated that this active small-molecule compound may have beneficial effects on improving glucose uptake. CONCLUSIONS: We envisage that network analysis will be useful in screening bioactive compounds of medicinal plants.
Authors: Máté Nagy-Pénzes; Zoltán Hajnády; Zsolt Regdon; Máté Á Demény; Katalin Kovács; Tarek El-Hamoly; József Maléth; Péter Hegyi; Csaba Hegedűs; László Virág Journal: Biomedicines Date: 2022-06-10
Authors: Chun Li; Xia Du; Yang Liu; Qi-Qi Liu; Wen Bing Zhi; Chun Liu Wang; Jie Zhou; Ye Li; Hong Zhang Journal: Evid Based Complement Alternat Med Date: 2020-01-08 Impact factor: 2.629