| Literature DB >> 31021473 |
Evgeny I Bakhmet1, Igor B Nazarov1, Adel R Gazizova1, Nadezhda E Vorobyeva2, Andrey A Kuzmin1, Mikhail N Gordeev1, Sergey A Sinenko1, Nikolai D Aksenov1, Tatyana O Artamonova3, Mikhail A Khodorkovskii3, Natalia Alenina4, Daria Onichtchouk5, Guangming Wu6, Hans R Schöler6, Alexey N Tomilin1,7.
Abstract
The transcription factor Oct4 plays a key regulatory role in the induction and maintenance of cellular pluripotency. In this article, we show that ubiquitous and multifunctional poly(C) DNA/RNA-binding protein hnRNP-K occupies Oct4 (Pou5f1) enhancers in embryonic stem cells (ESCs) but is dispensable for the initiation, maintenance, and downregulation of Oct4 gene expression. Nevertheless, hnRNP-K has an essential cell-autonomous function in ESCs to maintain their proliferation and viability. To better understand mechanisms of hnRNP-K action in ESCs, we have performed ChIP-seq analysis of genome-wide binding of hnRNP-K and identified several thousands of hnRNP-K target sites that are frequently co-occupied by pluripotency-related and common factors (Oct4, TATA-box binding protein, Sox2, Nanog, Otx2, etc.), as well as active histone marks. Furthermore, hnRNP-K localizes exclusively within open chromatin, implying its role in the onset and/or maintenance of this chromatin state. Stem Cells 2019;37:1018-1029. © AlphaMed Press 2019.Entities:
Keywords: Chromatin; Histone code; Oct4; Pluripotent stem cells; Pou5f1; hnRNP-K
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Year: 2019 PMID： 31021473 DOI： 10.1002/stem.3025
Source DB: PubMed Journal: Stem Cells ISSN： 1066-5099 Impact factor: 6.277