Jennifer L Spiegel1, Mario Hambrecht2, Vera Kohlbauer1, Frank Haubner1, Friedrich Ihler1,3, Martin Canis1, Arndt F Schilling4, Kai O Böker4, Ralf Dressel5, Katrin Streckfuss-Bömeke6,7, Mark Jakob1. 1. Department of Otorhinolaryngology, Klinikum der Universitaet Muenchen, Ludwig-Maximilians-Universitaet Muenchen, Munich, Germany. 2. Department of Otorhinolaryngology, Universitaetsmedizin Goettingen, University Medical Center Goettingen, Goettingen, Germany. 3. German Center for Vertigo and Balance Disorders, Klinikum der Universitaet Muenchen, Ludwig-Maximilians-Universitaet Muenchen, Munich, Germany. 4. Department of Trauma Surgery, Orthopedics and Plastic Surgery, University Medical Center Goettingen, Goettingen, Germany. 5. Institute of Cellular and Molecular Immunology, University Medical Center Goettingen, Goettingen, Germany. 6. Department of Cardiology and Pneumology, University Medical Center Goettingen, Goettingen, Germany. 7. DZHK (German Center for Cardiovascular Research), Partner Site Goettingen, Goettingen, Germany.
Abstract
BACKGROUND: Tissue-resident mesenchymal stem cells (MSCs) possess the ability to migrate to areas of inflammation and promote the regeneration of damaged tissue. However, it remains unclear how radiation influences this capacity of MSC in the head and neck region. METHODS: Two types of MSCs of the head and neck region (mucosa [mMSC] and parotid gland [pMSC]) were isolated, cultured and exposed to single radiation dosages of 2 Gy/day up to 10 days. Effects on morphology, colony forming ability, apoptosis, chemokine receptor expression, cytokine secretion, and cell migration were analyzed. RESULTS: Although MSC preserved MSC-specific regenerative abilities and immunomodulatory properties following irradiation in our in vitro model, we found a deleterious impact on colony forming ability, especially in pMSC. CONCLUSIONS: MSC exhibited robustness and activation upon radiation for the support of tissue regeneration, but lost their potential to replicate, thus possibly leading to depletion of the local MSC-pool after irradiation.
BACKGROUND: Tissue-resident mesenchymal stem cells (MSCs) possess the ability to migrate to areas of inflammation and promote the regeneration of damaged tissue. However, it remains unclear how radiation influences this capacity of MSC in the head and neck region. METHODS: Two types of MSCs of the head and neck region (mucosa [mMSC] and parotid gland [pMSC]) were isolated, cultured and exposed to single radiation dosages of 2 Gy/day up to 10 days. Effects on morphology, colony forming ability, apoptosis, chemokine receptor expression, cytokine secretion, and cell migration were analyzed. RESULTS: Although MSC preserved MSC-specific regenerative abilities and immunomodulatory properties following irradiation in our in vitro model, we found a deleterious impact on colony forming ability, especially in pMSC. CONCLUSIONS: MSC exhibited robustness and activation upon radiation for the support of tissue regeneration, but lost their potential to replicate, thus possibly leading to depletion of the local MSC-pool after irradiation.
Authors: Carl Grim; Robert Noble; Gabriela Uribe; Kamil Khanipov; Paul Johnson; Walter A Koltun; Tammara Watts; Yuriy Fofanov; Gregory S Yochum; Don W Powell; Ellen J Beswick; Irina V Pinchuk Journal: J Crohns Colitis Date: 2021-08-02 Impact factor: 9.071
Authors: Mark Jakob; Mario Hambrecht; Jennifer L Spiegel; Julia Kitz; Martin Canis; Ralf Dressel; Katrin Streckfuss-Bömeke Journal: Cells Date: 2020-12-28 Impact factor: 6.600