Literature DB >> 3101668

An active-site titrant for human tissue-type plasminogen activator.

R A Smith.   

Abstract

The reaction of recombinant tissue-type plasminogen activator with the inverse substrate 4-amidino-2-nitrophenyl 4'-anisate results in the rapid release of the chromogen 4-amidino-2-nitrophenol and the accumulation of the relatively stable 4-anisoyl-enzyme. Spectrophotometric monitoring of the reaction enables the operational molarity of the enzyme to be determined.

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Year:  1986        PMID： 3101668      PMCID： PMC1147305          DOI： 10.1042/bj2390477

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  18 in total

1.  p-Nitrophenyl-p'-guanidinobenzoate HCl: a new active site titrant for trypsin.

Authors:  T Chase; E Shaw
Journal:  Biochem Biophys Res Commun       Date:  1967-11-30       Impact factor: 3.575

2.  Alpha-chymotrypsin: enzyme concentration and kinetics.

Authors:  M L Bender; F J Kézdy; F C Wedler
Journal:  J Chem Educ       Date:  1967-02       Impact factor: 2.979

3.  Turnover of human extrinsic (tissue-type) plasminogen activator in rabbits.

Authors:  C Korninger; J M Stassen; D Collen
Journal:  Thromb Haemost       Date:  1981-10       Impact factor: 5.249

4.  Kinetics of the activation of plasminogen by human tissue plasminogen activator. Role of fibrin.

Authors:  M Hoylaerts; D C Rijken; H R Lijnen; D Collen
Journal:  J Biol Chem       Date:  1982-03-25       Impact factor: 5.157

5.  Thrombolysis by human tissue plasminogen activator and urokinase in rabbits with experimental pulmonary embolus.

Authors:  O Matsuo; D C Rijken; D Collen
Journal:  Nature       Date:  1981-06-18       Impact factor: 49.962

6.  Acyl-enzymes as thrombolytic agents in a rabbit model of venous thrombosis.

Authors:  R A Smith; R J Dupe; P D English; J Green
Journal:  Thromb Haemost       Date:  1982-06-28       Impact factor: 5.249

7.  Thrombolysis with human extrinsic (tissue-type) plasminogen activator in dogs with femoral vein thrombosis.

Authors:  C Korninger; O Matsuo; R Suy; J M Stassen; D Collen
Journal:  J Clin Invest       Date:  1982-03       Impact factor: 14.808

8.  Thrombolysis with human extrinsic (tissue-type) plasminogen activator in rabbits with experimental jugular vein thrombosis. Effect of molecular form and dose of activator, age of the thrombus, and route of administration.

Authors:  D Collen; J M Stassen; M Verstraete
Journal:  J Clin Invest       Date:  1983-02       Impact factor: 14.808

9.  Fibrinolysis with acyl-enzymes: a new approach to thrombolytic therapy.

Authors:  R A Smith; R J Dupe; P D English; J Green
Journal:  Nature       Date:  1981-04-09       Impact factor: 49.962

10.  A study of proteases and protease-inhibitor complexes in biological fluids.

Authors:  A Granelli-Piperno; E Reich
Journal:  J Exp Med       Date:  1978-07-01       Impact factor: 14.307
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  2 in total

1.  The interaction of streptokinase.plasminogen activator complex, tissue-type plasminogen activator, urokinase and their acylated derivatives with fibrin and cyanogen bromide digest of fibrinogen. Relationship to fibrinolytic potency in vitro.

Authors:  R Cassels; R Fears; R A Smith
Journal:  Biochem J       Date:  1987-10-15       Impact factor: 3.857

2.  Active site labeling with dansyl-glutamyl-glycyl-arginyl chloromethyl ketone demonstrates the full activity of the refolded and purified tissue-type plasminogen activator variant BM 06.022.

Authors:  U Kohnert; M Wozny; M Llinas; A Roos; S Fischer
Journal:  Appl Biochem Biotechnol       Date:  1995-11       Impact factor: 2.926
  2 in total

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