Daniela Antunes Montani1, Daniela Paes de Almeida Ferreira Braga2,3, Edson Borges4, Mariana Camargo1, Fernanda Bertuccez Cordeiro1,5, Eduardo Jorge Pilau6, Fábio Cesar Gozzo7, Renato Fraietta1, Edson Guimarães Lo Turco1. 1. Department of Surgery, Division of Urology, Human Reproduction Section, Sao Paulo Federal University, Rua Embau, 231, CEP: 04039-060, Sao Paulo, SP, Brazil. 2. Department of Surgery, Division of Urology, Human Reproduction Section, Sao Paulo Federal University, Rua Embau, 231, CEP: 04039-060, Sao Paulo, SP, Brazil. dbraga@fertility.com.br. 3. Fertility Medical Group, Av. Brigadeiro Luiz Antônio, 4545, CEP: 04511-010, Sao Paulo, SP, Brazil. dbraga@fertility.com.br. 4. Fertility Medical Group, Av. Brigadeiro Luiz Antônio, 4545, CEP: 04511-010, Sao Paulo, SP, Brazil. 5. Laboratorio para Investigaciones Biomédicas, Escuela Superior Politécnica del Litoral, ESPOL, Km. 30.5 Via Perimetral, CEP: 09-01-5863, Guayaquil, Ecuador. 6. Department of Chemistry, Maringa State University, Avenida Colombo, 5790, CEP: 87020-900, Maringá, PR, Brazil. 7. Institute of Chemistry, University of Campinas, R. Josué de Castro, 126, CEP: 13083-861, Campinas, SP, Brazil.
Abstract
PURPOSE: The present study aimed to provide a non-invasive approach to studying mechanisms responsible for oocyte development. METHODS: To this end, follicular fluid (FF) from 62 patients undergoing in vitro fertilization (IVF) cycles was split into two groups depending on the pregnancy outcome: pregnant (n = 28) and non-pregnant (n = 34) groups. Data were acquired by the MALDI-TOF mass spectrometry. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were applied to the data set. A ROC curve, to predict success rate, was constructed, and the lipids were attributed. RESULTS: Six ions were differentially represented in FF of pregnant and non-pregnant patients, with an area under the curve of 0.962. Phosphatidic acid, phosphatidylglycerol, and triacylglycerol were hyper-represented in the pregnant group, while glucosylceramide was hyper-represented in the non-pregnant group. Enriched functions related to these lipids are steroidogenesis, cellular response, signal transduction, cell cycle, and activation of protein kinase C for the pregnant group and apoptosis inhibition for the non-pregnant group. CONCLUSION: Human FF fingerprinting can both improve the understanding concerning mechanisms responsible for oocyte development and its effect on embryo implantation potential and assist in the management of IVF cycles.
PURPOSE: The present study aimed to provide a non-invasive approach to studying mechanisms responsible for oocyte development. METHODS: To this end, follicular fluid (FF) from 62 patients undergoing in vitro fertilization (IVF) cycles was split into two groups depending on the pregnancy outcome: pregnant (n = 28) and non-pregnant (n = 34) groups. Data were acquired by the MALDI-TOF mass spectrometry. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were applied to the data set. A ROC curve, to predict success rate, was constructed, and the lipids were attributed. RESULTS: Six ions were differentially represented in FF of pregnant and non-pregnant patients, with an area under the curve of 0.962. Phosphatidic acid, phosphatidylglycerol, and triacylglycerol were hyper-represented in the pregnant group, while glucosylceramide was hyper-represented in the non-pregnant group. Enriched functions related to these lipids are steroidogenesis, cellular response, signal transduction, cell cycle, and activation of protein kinase C for the pregnant group and apoptosis inhibition for the non-pregnant group. CONCLUSION:Human FF fingerprinting can both improve the understanding concerning mechanisms responsible for oocyte development and its effect on embryo implantation potential and assist in the management of IVF cycles.
Entities:
Keywords:
Follicular fluid; Lipidomics; Mass spectrometry; Metabolomics; Pregnancy
Authors: J L M R Leroy; T Vanholder; J R Delanghe; G Opsomer; A Van Soom; P E J Bols; J Dewulf; A de Kruif Journal: Theriogenology Date: 2004-09-15 Impact factor: 2.740