| Literature DB >> 31006651 |
Seung-Sik Rho1, Isao Kobayashi2, Eri Oguri-Nakamura1, Koji Ando3, Masakazu Fujiwara1, Naomi Kamimura4, Hiromi Hirata5, Atsuo Iida6, Yoshiko Iwai4, Naoki Mochizuki3, Shigetomo Fukuhara7.
Abstract
Hematopoietic stem cells (HSCs) emerge from hemogenic endothelium (HE) within the ventral portion of the dorsal aorta during vertebrate development. In zebrafish, Notch signaling induces HE specification from posterior lateral plate mesoderm (PLPM) cells as they migrate over the ventral surface of the somite. During migration, PLPM cells make close contact with Notch-ligand-expressing somitic cells to acquire HE identity. Herein, we show in zebrafish that the small GTPase Rap1b regulates HSC development by potentiating Notch-mediated HE specification. PLPM cells migrate toward the midline along the somite boundary where fibronectin accumulates. Rap1b stimulates integrin β1 to enhance PLPM cell adhesion to fibronectin localized at the somite boundary. Rap1b-induced integrin-β1-mediated adhesion to fibronectin leads to the spreading of PLPM cells to facilitate their physical contact with the Notch-ligand-expressing somitic cells, thereby promoting Notch-mediated HE specification. Thus, we have revealed an unexpected role of Rap1-induced integrin-mediated cell adhesion in HSC development.Entities:
Keywords: Notch; Rap1; fibronectin; hematopoietic stem cell; hemogenic endothelium; integrin β1; migration; posterior lateral plate mesoderm; somite; zebrafish
Year: 2019 PMID： 31006651 DOI： 10.1016/j.devcel.2019.03.023
Source DB: PubMed Journal: Dev Cell ISSN： 1534-5807 Impact factor: 12.270