OBJECTIVE: Connective tissue growth factor (CTGF) is an important regulator of fibrogenesis in many organs. This study evaluated the interrelationship among adipose tissue CTGF expression, fat mass, and insulin resistance in humans. METHODS: This study examined (1) CTGF gene expression in human subcutaneous preadipocytes before and after inducing adipogenesis; (2) relationships among abdominal subcutaneous adipose tissue CTGF gene expression, body fat mass, and indices of insulin sensitivity, including the hepatic insulin sensitivity index and the hyperinsulinemic-euglycemic clamp procedure in conjunction with stable isotope glucose tracer infusion, in 72 people who had marked differences in adiposity and insulin sensitivity; (3) localization of CTGF protein in subcutaneous adipose tissue; and (4) effect of progressive (5%, 11%, and 16%) weight loss on adipose tissue CTGF gene expression. RESULTS: CTGF was highly expressed in preadipocytes, not adipocytes. Adipose tissue CTGF expression was strongly correlated with body fat mass and both skeletal muscle and liver insulin sensitivity, and CTGF-positive cells were predominantly found in areas of fibrosis. Progressive weight loss caused a stepwise decrease in adipose tissue CTGF expression. CONCLUSIONS: It was concluded that increased CTGF expression is associated with adipose tissue expansion, adipose tissue fibrosis, and multi-organ insulin resistance in people with obesity.
OBJECTIVE:Connective tissue growth factor (CTGF) is an important regulator of fibrogenesis in many organs. This study evaluated the interrelationship among adipose tissue CTGF expression, fat mass, and insulin resistance in humans. METHODS: This study examined (1) CTGF gene expression in human subcutaneous preadipocytes before and after inducing adipogenesis; (2) relationships among abdominal subcutaneous adipose tissue CTGF gene expression, body fat mass, and indices of insulin sensitivity, including the hepatic insulin sensitivity index and the hyperinsulinemic-euglycemic clamp procedure in conjunction with stable isotope glucose tracer infusion, in 72 people who had marked differences in adiposity and insulin sensitivity; (3) localization of CTGF protein in subcutaneous adipose tissue; and (4) effect of progressive (5%, 11%, and 16%) weight loss on adipose tissue CTGF gene expression. RESULTS:CTGF was highly expressed in preadipocytes, not adipocytes. Adipose tissue CTGF expression was strongly correlated with body fat mass and both skeletal muscle and liver insulin sensitivity, and CTGF-positive cells were predominantly found in areas of fibrosis. Progressive weight loss caused a stepwise decrease in adipose tissue CTGF expression. CONCLUSIONS: It was concluded that increased CTGF expression is associated with adipose tissue expansion, adipose tissue fibrosis, and multi-organ insulin resistance in people with obesity.
Authors: Zhenyue Tong; Ruju Chen; Daniel S Alt; Sherri Kemper; Bernard Perbal; David R Brigstock Journal: Hepatology Date: 2009-09 Impact factor: 17.425
Authors: V Lokesh Battula; Ye Chen; Maria da Graca Cabreira; Vivian Ruvolo; Zhiqiang Wang; Wencai Ma; Sergej Konoplev; Elizabeth Shpall; Karen Lyons; Dirk Strunk; Carlos Bueso-Ramos; Richard Eric Davis; Marina Konopleva; Michael Andreeff Journal: Blood Date: 2013-06-05 Impact factor: 22.113
Authors: Magdalena Pasarica; Barbara Gowronska-Kozak; David Burk; Isabel Remedios; David Hymel; Jeff Gimble; Eric Ravussin; George A Bray; Steven R Smith Journal: J Clin Endocrinol Metab Date: 2009-10-16 Impact factor: 5.958
Authors: Elisa Fabbrini; Faidon Magkos; B Selma Mohammed; Terri Pietka; Nada A Abumrad; Bruce W Patterson; Adewole Okunade; Samuel Klein Journal: Proc Natl Acad Sci U S A Date: 2009-08-24 Impact factor: 11.205
Authors: H Yokoi; M Mukoyama; K Mori; M Kasahara; T Suganami; K Sawai; T Yoshioka; Y Saito; Y Ogawa; T Kuwabara; A Sugawara; K Nakao Journal: Kidney Int Date: 2007-12-12 Impact factor: 10.612
Authors: Joanne T M Tan; Susan V McLennan; William W Song; Lisa W-Y Lo; James G Bonner; Paul F Williams; Stephen M Twigg Journal: Am J Physiol Cell Physiol Date: 2008-07-02 Impact factor: 4.249
Authors: Joanne T M Tan; Susan V McLennan; Paul F Williams; Alireza Rezaeizadeh; Lisa W-Y Lo; James G Bonner; Stephen M Twigg Journal: Am J Physiol Endocrinol Metab Date: 2013-04-09 Impact factor: 4.310
Authors: Ozlem Senol-Cosar; Rachel J Roth Flach; Marina DiStefano; Anil Chawla; Sarah Nicoloro; Juerg Straubhaar; Olga T Hardy; Hye Lim Noh; Jason K Kim; Martin Wabitsch; Philipp E Scherer; Michael P Czech Journal: Nat Commun Date: 2016-02-16 Impact factor: 14.919
Authors: Joseph W Beals; Gordon I Smith; Mahalakshmi Shankaran; Anja Fuchs; George G Schweitzer; Jun Yoshino; Tyler Field; Marcy Matthews; Edna Nyangau; Darya Morozov; Bettina Mittendorfer; Marc K Hellerstein; Samuel Klein Journal: Hepatology Date: 2021-06-22 Impact factor: 17.425
Authors: Michael P Franczyk; Nathan Qi; Kelly L Stromsdorfer; Chengcheng Li; Shintaro Yamaguchi; Hiroshi Itoh; Mihoko Yoshino; Yo Sasaki; Rita T Brookheart; Brian N Finck; Brian J DeBosch; Samuel Klein; Jun Yoshino Journal: Endocrinology Date: 2021-03-01 Impact factor: 4.736
Authors: Jing Ren; Xiaoyu Wang; Sarah N Parry; Christine Yee; Mark D Gorrell; Susan V McLennan; Stephen M Twigg Journal: J Cell Commun Signal Date: 2022-01-17 Impact factor: 5.908