Erofili Papadopoulou1, Ourania Nicolatou-Galitis2, Ioannis Papassotiriou3, Helena Linardou4, Aikaterini Karagianni5, Konstantinos Tsixlakis5, Anthi Tarampikou6, Kelly Michalakakou3, Emmanouil Vardas2, Dimitrios Bafaloukos6. 1. Clinic of Hospital Dentistry, Dental School, National and Kapodistrian University of Athens, 2 Thivon street, Goudi, Athens, Greece. erofilpa@hotmail.com. 2. Clinic of Hospital Dentistry, Dental School, National and Kapodistrian University of Athens, 2 Thivon street, Goudi, Athens, Greece. 3. Department of Clinical Biochemistry, 'Aghia Sophia' Children's Hospital, Thivon and Papadiamantopoulou street, Goudi, Athens, Greece. 4. Oncology Unit, 'Metropolitan Hospital', 9 Ethnarxou Makariou Avenue, Athens, Greece. 5. Clinic of Oral Diagnosis and Radiology, Dental School, National and Kapodistrian University of Athens, 2 Thivon street, Goudi, Athens, Greece. 6. 1st Oncology Department, 'Metropolitan Hospital', 9 Ethnarxou Makariou Avenue, Piraeus, Greece.
Abstract
PURPOSE: Crevicular fluid was used to assess interleukin-17 (IL-17) and vascular endothelial growth factor (VEGF) in cancer patients receiving zoledronic acid and/or bevacizumab. The markers were also assessed in the serum. METHODS: Twenty-five patients were included and comprised three groups: patients who received zoledronic acid (n = 9), patients who received bevacizumab (n = 9), and patients who received zoledronic acid combined with bevacizumab (n = 5). One patient received zoledronic acid and everolimus and another received zoledronic acid, bevacizumab, and temsirolimus. IL-17 and VEGF were measured by standard quantitative ELISA kits and assessed in two study points. RESULTS: Twenty-four patients maintained good periodontal health; one had asymptomatic osteonecrosis of the jaw. First assessment: 44 samples were collected; 21 from serum and 23 from crevicular fluid. Second assessment, 6 months later: 11 samples were collected; 6 from serum and 5 from crevicular fluid. IL-17 was detected in all samples, in serum and crevicular fluid, and remained unchanged at both time points. Serum VEGF in patients with bevacizumab alone or combined with zoledronic acid was significantly lower compared with that of patients who received zoledronic acid alone. VEGF was not detected in the crevicular fluid. CONCLUSIONS: Crevicular fluid might be an easy, non-invasive means to assess IL-17. The stable values of IL-17 in crevicular fluid and serum and the lack of VEGF in the crevicular fluid could be related to the good periodontal health of our patients. Further studies are needed to assess IL-17 and VEGF in the crevicular fluid in patients with and without periodontal disease.
PURPOSE: Crevicular fluid was used to assess interleukin-17 (IL-17) and vascular endothelial growth factor (VEGF) in cancer patients receiving zoledronic acid and/or bevacizumab. The markers were also assessed in the serum. METHODS: Twenty-five patients were included and comprised three groups: patients who received zoledronic acid (n = 9), patients who received bevacizumab (n = 9), and patients who received zoledronic acid combined with bevacizumab (n = 5). One patient received zoledronic acid and everolimus and another received zoledronic acid, bevacizumab, and temsirolimus. IL-17 and VEGF were measured by standard quantitative ELISA kits and assessed in two study points. RESULTS: Twenty-four patients maintained good periodontal health; one had asymptomatic osteonecrosis of the jaw. First assessment: 44 samples were collected; 21 from serum and 23 from crevicular fluid. Second assessment, 6 months later: 11 samples were collected; 6 from serum and 5 from crevicular fluid. IL-17 was detected in all samples, in serum and crevicular fluid, and remained unchanged at both time points. Serum VEGF in patients with bevacizumab alone or combined with zoledronic acid was significantly lower compared with that of patients who received zoledronic acid alone. VEGF was not detected in the crevicular fluid. CONCLUSIONS: Crevicular fluid might be an easy, non-invasive means to assess IL-17. The stable values of IL-17 in crevicular fluid and serum and the lack of VEGF in the crevicular fluid could be related to the good periodontal health of our patients. Further studies are needed to assess IL-17 and VEGF in the crevicular fluid in patients with and without periodontal disease.
Authors: Natasha C Ajuz; Henrique Antunes; Thais A Mendonça; Fábio R Pires; José F Siqueira; Luciana Armada Journal: J Endod Date: 2014-07-16 Impact factor: 4.171
Authors: Bruce W Kirkham; Marissa N Lassere; John P Edmonds; Katherine M Juhasz; Paul A Bird; C Soon Lee; Ron Shnier; Ian J Portek Journal: Arthritis Rheum Date: 2006-04