Development and utilization of gyrA and gyrB gene-based diagnostics for the phytoplasma classified under 16Sr I group in plants and insects.

In the present study, a new set of primers of gyrA and gyrB genes of the phytoplasma genome were designed and validated for the successful detection and taxonomic classification of the previously identified phytoplasma strains of 'Candidatus P. asteris' (16SrI-B subgroup) associated with Catharanthus leaf yellows, sesame phyllody and the leafhopper (Hishimonus phycitis). Our results suggested the ability and sensitivity of gyrA and gyrB genes as an alternative molecular marker to identify the Ca. P. asteris strain up to subgroup level associated both with plants and insects.