Literature DB >> 30988149

Application of CRISPR/Cas9-Based Genome Editing in Studying the Mechanism of Pandrug Resistance in Klebsiella pneumoniae.

Qiaoling Sun1, Yu Wang2, Ning Dong3,4, Lingwei Shen1, Hongwei Zhou1, Yanyan Hu1, Danxia Gu5, Sheng Chen3,4, Rong Zhang6, Quanjiang Ji7.   

Abstract

In this study, a CRISPR/Cas9-mediated genome editing method was used to study the functions of the mgrB, tetA, and ramR genes in mediating colistin and tigecycline resistance in carbapenem-resistant Klebsiella pneumoniae (CRKP). Inactivation of the tetA or ramR gene or the mgrB gene by CRISPR/Cas9 affected bacterial susceptibility to tigecycline or colistin, respectively. This study proved that the CRISPR/Cas9-based genome editing method could be effectively applied to K. pneumoniae and should be further utilized for genetic characterization.
Copyright © 2019 American Society for Microbiology.

Entities:  

Keywords:  CRISPR/Cas9; Klebsiella pneumoniaezzm321990; drug resistance mechanisms; genome editing; pandrug resistance

Mesh：

Substances：

Year:  2019        PMID： 30988149      PMCID： PMC6591634          DOI： 10.1128/AAC.00113-19

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  19 in total

1.  CRISPR-Cas9 and CRISPR-Assisted Cytidine Deaminase Enable Precise and Efficient Genome Editing in Klebsiella pneumoniae.

Authors:  Yu Wang; Shanshan Wang; Weizhong Chen; Liqiang Song; Yifei Zhang; Zhen Shen; Fangyou Yu; Min Li; Quanjiang Ji
Journal:  Appl Environ Microbiol       Date:  2018-11-15       Impact factor: 4.792

2.  Highly Tigecycline-Resistant Klebsiella pneumoniae Sequence Type 11 (ST11) and ST147 Isolates from Companion Animals.

Authors:  Cristina M Ovejero; Jose Antonio Escudero; Daniel Thomas-Lopez; Andreas Hoefer; Gabriel Moyano; Natalia Montero; Carmen Martin-Espada; Bruno Gonzalez-Zorn
Journal:  Antimicrob Agents Chemother       Date:  2017-05-24       Impact factor: 5.191

3.  Influence of transcriptional activator RamA on expression of multidrug efflux pump AcrAB and tigecycline susceptibility in Klebsiella pneumoniae.

Authors:  Alexey Ruzin; Melissa A Visalli; David Keeney; Patricia A Bradford
Journal:  Antimicrob Agents Chemother       Date:  2005-03       Impact factor: 5.191

Review 4.  Klebsiella pneumoniae: a major worldwide source and shuttle for antibiotic resistance.

Authors:  Shiri Navon-Venezia; Kira Kondratyeva; Alessandra Carattoli
Journal:  FEMS Microbiol Rev       Date:  2017-05-01       Impact factor: 16.408

5.  Genomic and transcriptomic analyses of colistin-resistant clinical isolates of Klebsiella pneumoniae reveal multiple pathways of resistance.

Authors:  Meredith S Wright; Yo Suzuki; Marcus B Jones; Steven H Marshall; Susan D Rudin; David van Duin; Keith Kaye; Michael R Jacobs; Robert A Bonomo; Mark D Adams
Journal:  Antimicrob Agents Chemother       Date:  2014-11-10       Impact factor: 5.191

Review 6.  Klebsiella pneumoniae as a key trafficker of drug resistance genes from environmental to clinically important bacteria.

Authors:  Kelly L Wyres; Kathryn E Holt
Journal:  Curr Opin Microbiol       Date:  2018-05-01       Impact factor: 7.934

7.  Amino Acid Substitutions of CrrB Responsible for Resistance to Colistin through CrrC in Klebsiella pneumoniae.

Authors:  Yi-Hsiang Cheng; Tzu-Lung Lin; Yi-Tsung Lin; Jin-Town Wang
Journal:  Antimicrob Agents Chemother       Date:  2016-05-23       Impact factor: 5.191

8.  Genomics of KPC-producing Klebsiella pneumoniae sequence type 512 clone highlights the role of RamR and ribosomal S10 protein mutations in conferring tigecycline resistance.

Authors:  Laura Villa; Claudia Feudi; Daniela Fortini; Aurora García-Fernández; Alessandra Carattoli
Journal:  Antimicrob Agents Chemother       Date:  2013-12-30       Impact factor: 5.191

9.  Tigecycline Susceptibility and Molecular Resistance Mechanisms Among Clinical Klebsiella pneumoniae Strains Isolated During Non-Tigecycline Treatment.

Authors:  Ruilian Li; Yujia Han; Yiheng Zhou; Zemin Du; Hao Wu; Jing Wang; Yang Chen
Journal:  Microb Drug Resist       Date:  2016-05-24       Impact factor: 3.431

10.  First emergence of acrAB and oqxAB mediated tigecycline resistance in clinical isolates of Klebsiella pneumoniae pre-dating the use of tigecycline in a Chinese hospital.

Authors:  Xue Zhong; Hongtao Xu; Dongke Chen; Haijian Zhou; Xin Hu; Gang Cheng
Journal:  PLoS One       Date:  2014-12-12       Impact factor: 3.240
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  4 in total

Review 1.  Heterologous Expression of Lignocellulose-Modifying Enzymes in Microorganisms: Current Status.

Authors:  Alberto Moura Mendes Lopes; Manoela Martins; Rosana Goldbeck
Journal:  Mol Biotechnol       Date:  2021-01-23       Impact factor: 2.695

Review 2.  Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections.

Authors:  Yuye Wu; Dheerendranath Battalapalli; Mohammed J Hakeem; Venkatarao Selamneni; Pengfei Zhang; Mohamed S Draz; Zhi Ruan
Journal:  J Nanobiotechnology       Date:  2021-12-04       Impact factor: 10.435

Review 3.  CRISPR-Cas, a Revolution in the Treatment and Study of ESKAPE Infections: Pre-Clinical Studies.

Authors:  Manuel González de Aledo; Mónica González-Bardanca; Lucía Blasco; Olga Pacios; Inés Bleriot; Laura Fernández-García; Melisa Fernández-Quejo; María López; Germán Bou; María Tomás
Journal:  Antibiotics (Basel)       Date:  2021-06-22

Review 4.  Emerging Strategies to Combat β-Lactamase Producing ESKAPE Pathogens.

Authors:  Corneliu Ovidiu Vrancianu; Irina Gheorghe; Elena-Georgiana Dobre; Ilda Czobor Barbu; Roxana Elena Cristian; Marcela Popa; Sang Hee Lee; Carmen Limban; Ilinca Margareta Vlad; Mariana Carmen Chifiriuc
Journal:  Int J Mol Sci       Date:  2020-11-12       Impact factor: 6.208
  4 in total

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