Literature DB >> 3098735

Construction and properties of an intracellular serine protease mutant of Bacillus subtilis.

L Band, D J Henner, M Ruppen.   

Abstract

An intracellular serine protease (ISP-1) mutant of Bacillus subtilis was created by introducing a frameshift into the coding region of the cloned gene. Intracellular protease activity in the mutant was very low, yet sporulation in both nutrient broth and minimal medium was normal. The rate of bulk protein turnover in the mutant was slightly slower than that in the wild-type strain. These results suggest that the gene for ISP-1 is not essential and that ISP-1 is not the major enzyme involved in protein turnover during sporulation.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 3098735      PMCID： PMC211792          DOI： 10.1128/jb.169.1.444-446.1987

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  19 in total

1.  Induction of sporulation in developmental mutants of Bacillus subtilis.

Authors:  E B Freese; N Vasantha; E Freese
Journal:  Mol Gen Genet       Date:  1979-02-16

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Characterization of a thermosensitive sporulation mutant of Bacillus subtilis affected in the structural gene of an intracellular protease.

Authors:  P Kerjan; E Keryer; J Szulmajster
Journal:  Eur J Biochem       Date:  1979-08-01

4.  Intracellular serine protease of Bacillus subtilis: sequence homology with extracellular subtilisins.

Authors:  A Y Strongin; L S Izotova; Z T Abramov; D I Gorodetsky; L M Ermakova; L A Baratova; L P Belyanova; V M Stepanov
Journal:  J Bacteriol       Date:  1978-03       Impact factor: 3.490

5.  Cloning and sequencing of the major intracellular serine protease gene of Bacillus subtilis.

Authors:  Y Koide; A Nakamura; T Uozumi; T Beppu
Journal:  J Bacteriol       Date:  1986-07       Impact factor: 3.490

6.  Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

Authors:  G M Wahl; M Stern; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

7.  Effects of mutational loss of specific intracellular proteases on the sporulation of Bacillus subtilis.

Authors:  J H Hageman; B C Carlton
Journal:  J Bacteriol       Date:  1973-05       Impact factor: 3.490

8.  Catabolic repression of bacterial sporulation.

Authors:  P Schaeffer; J Millet; J P Aubert
Journal:  Proc Natl Acad Sci U S A       Date:  1965-09       Impact factor: 11.205

Review 9.  Proteolysis in bacterial sporulation.

Authors:  M R Maurizi; R L Switzer
Journal:  Curr Top Cell Regul       Date:  1980

Review 10.  The inactivation of microbial enzymes in vivo.

Authors:  R L Switzer
Journal:  Annu Rev Microbiol       Date:  1977       Impact factor: 15.500
View more
  7 in total

1.  Intracellular serine protease 1 of Bacillus subtilis is formed in vivo as an unprocessed, active protease in stationary cells.

Authors:  S M Sheehan; R L Switzer
Journal:  J Bacteriol       Date:  1990-01       Impact factor: 3.490

2.  Intracellular serine protease-4, a new intracellular serine protease activity from Bacillus subtilis.

Authors:  S M Sheehan; R L Switzer
Journal:  Arch Microbiol       Date:  1991       Impact factor: 2.552

3.  Energy and calcium ion dependence of proteolysis during sporulation of Bacillus subtilis cells.

Authors:  M B O'Hara; J H Hageman
Journal:  J Bacteriol       Date:  1990-08       Impact factor: 3.490

4.  Control of intracellular serine protease expression in Bacillus subtilis.

Authors:  M E Ruppen; G L Van Alstine; L Band
Journal:  J Bacteriol       Date:  1988-01       Impact factor: 3.490

5.  Characterization of the gene encoding an intracellular proteinase inhibitor of Bacillus subtilis and its role in regulation of the major intracellular proteinase.

Authors:  Y Shiga; H Yamagata; S Udaka
Journal:  J Bacteriol       Date:  1993-11       Impact factor: 3.490

6.  Immunocytochemical localization of a calmodulinlike protein in Bacillus subtilis cells.

Authors:  D C Dominguez; H Adams; J H Hageman
Journal:  J Bacteriol       Date:  1999-08       Impact factor: 3.490

7.  The first structure in a family of peptidase inhibitors reveals an unusual Ig-like fold.

Authors:  Daniel J Rigden; Qingping Xu; Yuanyuan Chang; Ruth Y Eberhardt; Robert D Finn; Neil D Rawlings
Journal:  F1000Res       Date:  2013-07-10
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.