Reciprocal stimulating effects of bFGF and FSH on chicken primordial follicle activation through AKT and ERK pathway.

Basic fibroblast growth factor (bFGF) and follicle-stimulating hormone (FSH) both play important roles in primordial follicle development. Here we investigated the reciprocal stimulation effects of a cytokine bFGF and FSH on primordial follicle development in the chicken and considered a possible signaling mechanism involving protein kinase B (AKT) and extracellular regulated protein kinase (ERK) pathways. 4-day-old chicken ovaries were treated with bFGF and FSH for 3 days in culture to investigate the effects of bFGF and FSH on primordial follicle development. Methods included HE staining, immunohistochemistry, quantitate real-time PCR, Western blot and immunofluorescence. A correlated change of bFGF receptor (FGFR1) mRNA expression and time course of primordial follicle activation was revealed in the early chick ovaries. A reciprocal stimulation effect on primordial follicle activation was demonstrated for bFGF and FSH, along with accelerated granulosa cells proliferation and decreased cell apoptosis. The promoting effect of bFGF was attenuated by the FGFR1 inhibitor SU5402 where the percentage of growing follicles had decreased. AKT and ERK signaling pathways mediated the action of bFGF and FSH in their promotion of primordial follicle activation. Cytokine bFGF and FSH imposed reciprocal stimulating effects on granulosa cell proliferation and anti-apoptosis to promote primordial follicle activation via the PI3K-AKT and ERK signaling pathways in early chick ovaries.