Zsolt Csenki1, Edina Garai2, Anita Risa3, Mátyás Cserháti3, Katalin Bakos2, Dalma Márton3, Zoltán Bokor2, Balázs Kriszt3, Béla Urbányi2. 1. Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences, Szent István University, 1. Páter Károly St., H-2100, Gödöllő, Hungary. Electronic address: csenki.zsolt@mkk.szie.hu. 2. Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences, Szent István University, 1. Páter Károly St., H-2100, Gödöllő, Hungary. 3. Department of Environmental Safety and Ecotoxicology, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences, Szent István University, 1. Páter Károly St., H-2100, Gödöllő, Hungary.
Abstract
The use of microinjection of newly fertilized zebrafish eggs as an appropriate tool for qualifying the biodetoxification properties of toxin-degrading microbes was investigated. Ochratoxin A (OTA), bacterial degradation products of OTA and bacterial metabolites of the Cupriavidus basilensis ŐR16 strain were microinjected. Results showed that variations in the injected droplet size, and thus treatment concentrations, stayed within ±20%, moreover embryo mortality did not exceed 10% in controls, that is in accordance with the recommendations of the OECD 236 guideline. The highest lethality was caused by OTA with a significantly higher toxicity than that of bacterial metabolites or OTA degradation products. However, toxicity of the latter two did not differ statistically from each other showing that the observed mortality was due to the intrinsic toxicity of bacterial metabolites (and not OTA degradation products), thus, the strain effectively degrades OTA to nontoxic products. Sublethal symptoms also confirmed this finding. RESULTS: confirmed that microinjection of zebrafish embryos could be a reliable tool for testing the toxin-degrading properties of microbes. The method also allows comparisons among microbial strains able to degrade the same toxin, helping the selection of effective and environmentally safe microbial strains for the biodetoxification of mycotoxins in large scale.
The use of microinjection of newly fertilized zebrafish eggs as an appropriate tool for qualifying the biodetoxification properties of toxin-degrading microbes was investigated. Ochratoxin A (OTA), bacterial degradation products of OTA and bacterial metabolites of the Cupriavidus basilensis ŐR16 strain were microinjected. Results showed that variations in the injected droplet size, and thus treatment concentrations, stayed within ±20%, moreover embryo mortality did not exceed 10% in controls, that is in accordance with the recommendations of the OECD 236 guideline. The highest lethality was caused by OTA with a significantly higher toxicity than that of bacterial metabolites or OTA degradation products. However, toxicity of the latter two did not differ statistically from each other showing that the observed mortality was due to the intrinsic toxicity of bacterial metabolites (and not OTA degradation products), thus, the strain effectively degrades OTA to nontoxic products. Sublethal symptoms also confirmed this finding. RESULTS: confirmed that microinjection of zebrafish embryos could be a reliable tool for testing the toxin-degrading properties of microbes. The method also allows comparisons among microbial strains able to degrade the same toxin, helping the selection of effective and environmentally safe microbial strains for the biodetoxification of mycotoxins in large scale.
Authors: Mohammed Al-Nussairawi; Anita Risa; Edina Garai; Emese Varga; István Szabó; Zsolt Csenki-Bakos; Balázs Kriszt; Mátyás Cserháti Journal: Curr Microbiol Date: 2020-06-05 Impact factor: 2.188
Authors: Edina Garai; Anita Risa; Emese Varga; Mátyás Cserháti; Balázs Kriszt; Béla Urbányi; Zsolt Csenki Journal: Int J Mol Sci Date: 2021-01-13 Impact factor: 5.923
Authors: Zsolt Csenki; Anita Risa; Dorottya Sárkány; Edina Garai; Ildikó Bata-Vidács; Erzsébet Baka; András Szekeres; Mónika Varga; András Ács; Jeffrey Griffitts; Katalin Bakos; Illés Bock; István Szabó; Balázs Kriszt; Béla Urbányi; József Kukolya Journal: Toxins (Basel) Date: 2022-03-31 Impact factor: 5.075