Literature DB >> 30984925

T7 exo-mediated FRET-breaking combined with DSN-RNAse-TdT for the detection of microRNA with ultrahigh signal-amplification.

Van Thang Nguyen1, Binh Huy Le, Young Jun Seo.   

Abstract

A DSN-RNAse-TdT-T7 exo probing system allows the detection of miRNA 21 with very high sensitivity (LOD = 2.57 fM) and selectivity-the result of (i) avoiding the false-positive signal from miRNA reacting with TdT polymerase and (ii) signal amplification occurring through a FRET-breaking mechanism involving T7 exo.

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Year:  2019        PMID： 30984925     DOI： 10.1039/c9an00303g

Source DB:  PubMed          Journal:  Analyst        ISSN： 0003-2654            Impact factor:   4.616


  2 in total

1.  Highly Sensitive Fluorescence Assay for miRNA Detection: Investigation of the DNA Spacer Effect on the DSN Enzyme Activity toward Magnetic-Bead-Tethered Probes.

Authors:  Khouloud Djebbi; Biao Shi; Ting Weng; Mohamed Bahri; Mohamed Amin Elaguech; Jin Liu; Chaker Tlili; Deqiang Wang
Journal:  ACS Omega       Date:  2022-01-07

2.  A rapid and reliable CE-LIF method for the quantitative analysis of miRNA-497 in plasma and organs and its application to a pharmacokinetic and biodistribution study.

Authors:  Eunmi Ban; Haejin Kwon; Eun Joo Song
Journal:  RSC Adv       Date:  2020-05-18       Impact factor: 4.036
  2 in total

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