Literature DB >> 3098235

Reconstitution of surfactant activity by using the 6 kDa apoprotein associated with pulmonary surfactant.

S H Yu, F Possmayer.   

Abstract

Lipid extracts of bovine pulmonary surfactant containing the 6 kDa apoprotein, but lacking the 35 kDa apoprotein, can mimic the essential characteristics of pulmonary surfactant on a pulsating-bubble surfactometer. Reconstituted surfactant can be produced by combining silicic acid fractions containing 6 kDa apoprotein and phosphatidylglycerol with phosphatidylcholine. Treatment of the protein-containing fraction with proteolytic enzymes abolishes its efficacy. These results indicate that the presence of the 6 kDa apoprotein can account for some of the essential physical and biological characteristics of pulmonary surfactant. Immunodiffusion studies indicate that, contrary to earlier suggestions, the 6 kDa apoprotein is not structurally related to the major surfactant apoprotein that has a molecular mass of 35 kDa.

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Year:  1986        PMID： 3098235      PMCID： PMC1146789          DOI： 10.1042/bj2360085

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  26 in total

1.  The physical properties of an effective lung surfactant.

Authors:  A D Bangham; C J Morley; M C Phillips
Journal:  Biochim Biophys Acta       Date:  1979-06-21

2.  Isolation and further characterization of the anti-encephalitogenic rat spinal cord protein from brain, spinal cord and peripheral nerves.

Authors:  C F MacPherson; H Armstrong; S H Yu
Journal:  Biochim Biophys Acta       Date:  1981-07-28

3.  Interaction of the lipid and protein components of pulmonary surfactant. Role of phosphatidylglycerol and calcium.

Authors:  R J King; M C MacBeth
Journal:  Biochim Biophys Acta       Date:  1981-10-02

4.  Pulmonary surfactant-associated proteins: their role in the expression of surface activity.

Authors:  I L Metcalfe; G Enhorning; F Possmayer
Journal:  J Appl Physiol Respir Environ Exerc Physiol       Date:  1980-07

5.  Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis.

Authors:  M W Hunkapiller; E Lujan; F Ostrander; L E Hood
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

6.  Surfactant substitution.

Authors:  M Obladen; F Brendlein; B Krempien
Journal:  Eur J Pediatr       Date:  1979-08       Impact factor: 3.183

7.  Calcium interactions in pulmonary surfactant.

Authors:  M J Weber; F Possmayer
Journal:  Biochim Biophys Acta       Date:  1984-10-24

8.  An ethanol/ether soluble apoprotein from rat lung surfactant augments liposome uptake by isolated granular pneumocytes.

Authors:  W D Claypool; D L Wang; A Chander; A B Fisher
Journal:  J Clin Invest       Date:  1984-09       Impact factor: 14.808

9.  Bovine pulmonary surfactant: chemical composition and physical properties.

Authors:  S Yu; P G Harding; N Smith; F Possmayer
Journal:  Lipids       Date:  1983-08       Impact factor: 1.880

10.  Experimental studies on the pulmonary surfactant. Reconstitution of surface-active material.

Authors:  Y Suzuki; E Nakai; K Ohkawa
Journal:  J Lipid Res       Date:  1982-01       Impact factor: 5.922
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  18 in total

1.  Prolonged ventilation of the premature newborn rabbit after treatment with natural or apoprotein-based artificial surfactant.

Authors:  B Robertson; T Curstedt; G Grossmann; T Kobayashi; M Kokubo; Y Suzuki
Journal:  Eur J Pediatr       Date:  1988-02       Impact factor: 3.183

2.  Regulation of messenger RNAs for the hydrophobic surfactant proteins in human lung.

Authors:  H G Liley; R T White; R G Warr; B J Benson; S Hawgood; P L Ballard
Journal:  J Clin Invest       Date:  1989-04       Impact factor: 14.808

3.  Biophysical and biological activity of a synthetic 8.7-kDa hydrophobic pulmonary surfactant protein SP-B.

Authors:  V K Sarin; S Gupta; T K Leung; V E Taylor; B L Ohning; J A Whitsett; J L Fox
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205

4.  Glucocorticoids both stimulate and inhibit production of pulmonary surfactant protein A in fetal human lung.

Authors:  H G Liley; R T White; B J Benson; P L Ballard
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

5.  Use of human surfactant low molecular weight apoproteins in the reconstitution of surfactant biologic activity.

Authors:  S D Revak; T A Merritt; E Degryse; L Stefani; M Courtney; M Hallman; C G Cochrane
Journal:  J Clin Invest       Date:  1988-03       Impact factor: 14.808

6.  cDNA and deduced amino acid sequence of human pulmonary surfactant-associated proteolipid SPL(Phe).

Authors:  S W Glasser; T R Korfhagen; T Weaver; T Pilot-Matias; J L Fox; J A Whitsett
Journal:  Proc Natl Acad Sci U S A       Date:  1987-06       Impact factor: 11.205

7.  Antigenicity of low molecular weight surfactant species.

Authors:  D S Strayer; T A Merritt; C Makunike; M Hallman
Journal:  Am J Pathol       Date:  1989-04       Impact factor: 4.307

8.  Low molecular weight human pulmonary surfactant protein (SP5): isolation, characterization, and cDNA and amino acid sequences.

Authors:  R G Warr; S Hawgood; D I Buckley; T M Crisp; J Schilling; B J Benson; P L Ballard; J A Clements; R T White
Journal:  Proc Natl Acad Sci U S A       Date:  1987-11       Impact factor: 11.205

9.  Isolation and chemical composition of surface-active material from human lung lavage.

Authors:  T Sadana; K Dhall; S N Sanyal; A Wali; R Minocha; S Majumdar
Journal:  Lipids       Date:  1988-06       Impact factor: 1.880

10.  Pulmonary surfactant protein SP-C causes packing rearrangements of dipalmitoylphosphatidylcholine in spread monolayers.

Authors:  J Pérez-Gil; K Nag; S Taneva; K M Keough
Journal:  Biophys J       Date:  1992-07       Impact factor: 4.033
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