David Ortega-Paredes1, Marco Haro2, Paula Leoro-Garzón2, Pedro Barba3, Karen Loaiza4, Francisco Mora5, Martha Fors6, Christian Vinueza-Burgos7, Esteban Fernández-Moreira6. 1. Carrera de Medicina, Facultad de Ciencias de la Salud, Universidad de las Américas, Quito, Ecuador; Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencias a los Antibióticos, Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito, Ecuador; Life Science Initiative, lsi-ec.com, Quito, Ecuador. Electronic address: daortegap@uce.edu.ec. 2. Life Science Initiative, lsi-ec.com, Quito, Ecuador; Laboratorio Clínico e Inmunológico INMUNOLAB, Quito, Ecuador. 3. Carrera de Ingeniería en Biotecnología, Facultad de Ingeniería en Ciencias Agropecuarias y Ambientales, Universidad Técnica del Norte, Ibarra, Ecuador. 4. Life Science Initiative, lsi-ec.com, Quito, Ecuador. 5. Carrera de Medicina, Facultad de Ciencias de la Salud, Universidad de las Américas, Quito, Ecuador; Hospital General del Sur de Quito (IESS), Quito, Ecuador. 6. Carrera de Medicina, Facultad de Ciencias de la Salud, Universidad de las Américas, Quito, Ecuador. 7. Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencias a los Antibióticos, Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito, Ecuador.
Abstract
OBJECTIVES: This study focused on estimating the prevalence of extended-spectrum β-lactamases (ESBLs), plasmid-mediated AmpC β-lactamases, carbapenemases and MCR-1-producing Escherichia coli in canine faeces from a public park in Quito, Ecuador. METHODS: Phenotypic and genotypic characterisation of E. coli isolated from 50 canine faecal samples recovered from a city park in Quito was performed. In addition, a multiple choice survey was conducted among 50 dog owners. RESULTS: Of the 50 faecal samples, 20 (40.0%) presented E. coli resistant to ceftriaxone. Moreover, 23 E. coli isolates were recovered for further analysis. All of the isolates showed as multidrug-resistant (MDR) phenotype (resistant to three or more antibiotic families). Resistance to carbapenems, tigecycline and amikacin was not observed. No major clonal relatedness was observed among the resistant isolates. The ESBL genes blaCTX-M-15, blaCTX-M-55 and blaCTX-M-65 were the most common. Two isolates harboured the blaCMY-2 gene and one isolate harboured both mcr-1 and blaCTX-M-65. Statistical analysis showed that older people were more conscious of collecting and disposing of dog faeces than subjects aged <35 years (P < 0.05). CONCLUSION: The finding of MDR E. coli in dog faeces in a city park in Ecuador illustrates the importance of analysing canine faeces in public settings (e.g. parks, playgrounds) as part of surveillance programmes for MDR E. coli. In addition, this research might be a sentinel sampling method to gain a better understanding of community sources of antibiotic-resistant Enterobacteriaceae at human-animal-environment interfaces.
OBJECTIVES: This study focused on estimating the prevalence of extended-spectrum β-lactamases (ESBLs), plasmid-mediated AmpC β-lactamases, carbapenemases and MCR-1-producing Escherichia coli in canine faeces from a public park in Quito, Ecuador. METHODS: Phenotypic and genotypic characterisation of E. coli isolated from 50 canine faecal samples recovered from a city park in Quito was performed. In addition, a multiple choice survey was conducted among 50 dog owners. RESULTS: Of the 50 faecal samples, 20 (40.0%) presented E. coli resistant to ceftriaxone. Moreover, 23 E. coli isolates were recovered for further analysis. All of the isolates showed as multidrug-resistant (MDR) phenotype (resistant to three or more antibiotic families). Resistance to carbapenems, tigecycline and amikacin was not observed. No major clonal relatedness was observed among the resistant isolates. The ESBL genes blaCTX-M-15, blaCTX-M-55 and blaCTX-M-65 were the most common. Two isolates harboured the blaCMY-2 gene and one isolate harboured both mcr-1 and blaCTX-M-65. Statistical analysis showed that older people were more conscious of collecting and disposing of dog faeces than subjects aged <35 years (P < 0.05). CONCLUSION: The finding of MDR E. coli in dog faeces in a city park in Ecuador illustrates the importance of analysing canine faeces in public settings (e.g. parks, playgrounds) as part of surveillance programmes for MDR E. coli. In addition, this research might be a sentinel sampling method to gain a better understanding of community sources of antibiotic-resistant Enterobacteriaceae at human-animal-environment interfaces.
Authors: Stella M Hartinger; Maria Luisa Medina-Pizzali; Gabriela Salmon-Mulanovich; Anika J Larson; María Pinedo-Bardales; Hector Verastegui; Maribel Riberos; Daniel Mäusezahl Journal: Int J Environ Res Public Health Date: 2021-04-27 Impact factor: 3.390
Authors: Julio A Benavides; Marília Salgado-Caxito; Andrés Opazo-Capurro; Paulina González Muñoz; Ana Piñeiro; Macarena Otto Medina; Lina Rivas; Jose Munita; Javier Millán Journal: Antibiotics (Basel) Date: 2021-04-30
Authors: Philip Joosten; Daniela Ceccarelli; Evelien Odent; Steven Sarrazin; Haitske Graveland; Liese Van Gompel; Antonio Battisti; Andrea Caprioli; Alessia Franco; Jaap A Wagenaar; Dik Mevius; Jeroen Dewulf Journal: Antibiotics (Basel) Date: 2020-02-16
Authors: David Ortega-Paredes; Sofía de Janon; Fernando Villavicencio; Katherine Jaramillo Ruales; Kenny De La Torre; José E Villacís; Jaap A Wagenaar; Jorge Matheu; Camila Bravo-Vallejo; Esteban Fernández-Moreira; Christian Vinueza-Burgos Journal: Front Vet Sci Date: 2020-11-25
Authors: Marília Salgado-Caxito; Andrea I Moreno-Switt; Antonio Carlos Paes; Carlos Shiva; Jose M Munita; Lina Rivas; Julio A Benavides Journal: Antibiotics (Basel) Date: 2021-01-28
Authors: Carlos Bastidas-Caldes; Daniel Romero-Alvarez; Victor Valdez-Vélez; Roberto D Morales; Andrés Montalvo-Hernández; Cicero Gomes-Dias; Manuel Calvopiña Journal: Infect Drug Resist Date: 2022-09-30 Impact factor: 4.177