Literature DB >> 30977478

5'-Morpholino modification of the sense strand of an siRNA makes it a more effective passenger.

Pawan Kumar1, Rubina G Parmar, Christopher R Brown, Jennifer L S Willoughby, Donald J Foster, I Ramesh Babu, Sally Schofield, Vasant Jadhav, Klaus Charisse, Jayaprakash K Nair, Kallanthottathil G Rajeev, Martin A Maier, Martin Egli, Muthiah Manoharan.   

Abstract

The 5'-monophosphate group plays an important role in strand selection during gene silencing mediated by small-interfering RNA. We show that blocking of 5' phosphorylation of the sense strand by introducing a 5'-morpholino modification improves antisense strand selection and RNAi activity. The 5'-morpholino modification of the antisense strand triggers complete loss of activity.

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Year:  2019        PMID： 30977478     DOI： 10.1039/c9cc00977a

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  8 in total

1.  Aminooxy Click Chemistry as a Tool for Bis-homo and Bis-hetero Ligand Conjugation to Nucleic Acids.

Authors:  Dhrubajyoti Datta; Shohei Mori; Mimouna Madaoui; Kelly Wassarman; Ivan Zlatev; Muthiah Manoharan
Journal:  Org Lett       Date:  2022-06-17       Impact factor: 6.072

2.  Amide-Modified RNA: Using Protein Backbone to Modulate Function of Short Interfering RNAs.

Authors:  Venubabu Kotikam; Eriks Rozners
Journal:  Acc Chem Res       Date:  2020-07-13       Impact factor: 22.384

3.  Investigation of Strand-Selective Interaction of SNA-Modified siRNA with AGO2-MID.

Authors:  Yukiko Kamiya; Yuuki Takeyama; Tomonari Mizuno; Fuminori Satoh; Hiroyuki Asanuma
Journal:  Int J Mol Sci       Date:  2020-07-23       Impact factor: 5.923

4.  Chitosan Oleate Coated PLGA Nanoparticles as siRNA Drug Delivery System.

Authors:  Dalila Miele; Xin Xia; Laura Catenacci; Milena Sorrenti; Silvia Rossi; Giuseppina Sandri; Franca Ferrari; John J Rossi; Maria Cristina Bonferoni
Journal:  Pharmaceutics       Date:  2021-10-17       Impact factor: 6.525

5.  Innovative developments and emerging technologies in RNA therapeutics.

Authors:  François Halloy; Annabelle Biscans; Katherine E Bujold; Alexandre Debacker; Alyssa C Hill; Aurélie Lacroix; Olivia Luige; Roger Strömberg; Linda Sundstrom; Jörg Vogel; Alice Ghidini
Journal:  RNA Biol       Date:  2021-12-31       Impact factor: 4.652

Review 6.  Chemical strategies for strand selection in short-interfering RNAs.

Authors:  Andrew J Varley; Jean-Paul Desaulniers
Journal:  RSC Adv       Date:  2021-01-11       Impact factor: 3.361

7.  Chimeric siRNAs with chemically modified pentofuranose and hexopyranose nucleotides: altritol-nucleotide (ANA) containing GalNAc-siRNA conjugates: in vitro and in vivo RNAi activity and resistance to 5'-exonuclease.

Authors:  Pawan Kumar; Rohan Degaonkar; Dale C Guenther; Mikhail Abramov; Guy Schepers; Marie Capobianco; Yongfeng Jiang; Joel Harp; Charalambos Kaittanis; Maja M Janas; Adam Castoreno; Ivan Zlatev; Mark K Schlegel; Piet Herdewijn; Martin Egli; Muthiah Manoharan
Journal:  Nucleic Acids Res       Date:  2020-05-07       Impact factor: 16.971

Review 8.  Effective tools for RNA-derived therapeutics: siRNA interference or miRNA mimicry.

Authors:  Peipei Wang; Yue Zhou; Arthur M Richards
Journal:  Theranostics       Date:  2021-08-11       Impact factor: 11.556
  8 in total

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