In vitro analysis of blood flow in a microvascular network with realistic geometry.

In vitro blood flow was measured in a polydimethysiloxane micro channel to reflect the complex geometry of a microvascular network. Flow rates were determined from the velocities of tracer particles moving along the center line of the flow channel, and the flow rates of two working fluids were then compared: water and blood. In some bifurcating channels, the measured flow rate showed that the effects of bifurcation in the apparent viscosity depend on the hematocrit, such that the flow rate in the daughter channel with the higher (lower) flow rate was lower (higher) for blood than for water. The measured flow rates in other bifurcating channels reflected effects from the surrounding flow channels acting as bypasses, which tended to balance out the effects of bifurcation.