Literature DB >> 3096954

Use of the Bacillus subtilis subtilisin signal peptide for efficient secretion of TEM beta-lactamase during growth.

S L Wong, F Kawamura, R H Doi.   

Abstract

We report the development of an efficient Bacillus subtilis secretory system, with the secreted product stably maintained in the medium for 100 h. The system is based on characterization of the subtilisin signal peptidase cleavage site and promoters, catabolite repression of sporulation, presence of a vegetative secreting mechanism, and availability of a protease-deficient strain.

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Year:  1986        PMID： 3096954      PMCID： PMC213584          DOI： 10.1128/jb.168.2.1005-1009.1986

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  16 in total

1.  Determination of the signal peptidase cleavage site in the preprosubtilisin of Bacillus subtilis.

Authors:  S L Wong; R H Doi
Journal:  J Biol Chem       Date:  1986-08-05       Impact factor: 5.157

2.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

3.  Catabolic repression of bacterial sporulation.

Authors:  P Schaeffer; J Millet; J P Aubert
Journal:  Proc Natl Acad Sci U S A       Date:  1965-09       Impact factor: 11.205

4.  Nucleotide sequence of the amylase gene from Bacillus subtilis.

Authors:  M Yang; A Galizzi; D Henner
Journal:  Nucleic Acids Res       Date:  1983-01-25       Impact factor: 16.971

5.  Production in B. subtilis of hepatitis B core antigen and a major antigen of foot and mouth disease virus.

Authors:  K Hardy; S Stahl; H Küpper
Journal:  Nature       Date:  1981-10-08       Impact factor: 49.962

6.  Zinc is associated with the beta subunit of DNA-dependent RNA polymerase of Bacillus subtilis.

Authors:  S M Halling; F J Sanchez-Anzaldo; R Fukuda; R H Doi; C F Meares
Journal:  Biochemistry       Date:  1977-06-28       Impact factor: 3.162

7.  Replacement of the Bacillus subtilis subtilisin structural gene with an In vitro-derived deletion mutation.

Authors:  M L Stahl; E Ferrari
Journal:  J Bacteriol       Date:  1984-05       Impact factor: 3.490

8.  The subtilisin E gene of Bacillus subtilis is transcribed from a sigma 37 promoter in vivo.

Authors:  S L Wong; C W Price; D S Goldfarb; R H Doi
Journal:  Proc Natl Acad Sci U S A       Date:  1984-02       Impact factor: 11.205

9.  Secretion of Escherichia coli beta-lactamase from Bacillus subtilis by the aid of alpha-amylase signal sequence.

Authors:  I Palva; M Sarvas; P Lehtovaara; M Sibakov; L Kääriäinen
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

10.  Secretion of interferon by Bacillus subtilis.

Authors:  I Palva; P Lehtovaara; L Kääriäinen; M Sibakov; K Cantell; C H Schein; K Kashiwagi; C Weissmann
Journal:  Gene       Date:  1983 May-Jun       Impact factor: 3.688
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  8 in total

1.  rpoD operon promoter used by sigma H-RNA polymerase in Bacillus subtilis.

Authors:  H L Carter; L F Wang; R H Doi; C P Moran
Journal:  J Bacteriol       Date:  1988-04       Impact factor: 3.490

Review 2.  Protein secretion in Bacillus species.

Authors:  M Simonen; I Palva
Journal:  Microbiol Rev       Date:  1993-03

3.  Secretory expression in Escherichia coli and Bacillus subtilis of human interferon alpha genes directed by staphylokinase signals.

Authors:  R Breitling; D Gerlach; M Hartmann; D Behnke
Journal:  Mol Gen Genet       Date:  1989-06

4.  Secretion of the alpha-galactosidase from Cyamopsis tetragonoloba (guar) by Bacillus subtilis.

Authors:  N Overbeeke; G H Termorshuizen; M L Giuseppin; D R Underwood; C T Verrips
Journal:  Appl Environ Microbiol       Date:  1990-05       Impact factor: 4.792

5.  Functional characterization and localization of a Bacillus subtilis sortase and its substrate and use of this sortase system to covalently anchor a heterologous protein to the B. subtilis cell wall for surface display.

Authors:  Pei Xiong Liew; Christopher L C Wang; Sui-Lam Wong
Journal:  J Bacteriol       Date:  2011-10-21       Impact factor: 3.490

6.  Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases.

Authors:  X C Wu; W Lee; L Tran; S L Wong
Journal:  J Bacteriol       Date:  1991-08       Impact factor: 3.490

7.  Inactivation of the major extracellular protease from Bacillus megaterium DSM319 by gene replacement.

Authors:  K D Wittchen; F Meinhardt
Journal:  Appl Microbiol Biotechnol       Date:  1995-03       Impact factor: 4.813

8.  Cloning and expression in Escherichia coli, Bacillus subtilis, and Streptococcus sanguis of a gene for staphylokinase--a bacterial plasminogen activator.

Authors:  D Behnke; D Gerlach
Journal:  Mol Gen Genet       Date:  1987-12
  8 in total

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