| Literature DB >> 30968361 |
Eleen Y Shum1, Elisabeth M Walczak2, Christina Chang2, H Christina Fan2.
Abstract
In this review, we describe the BD Rhapsody™ Single-Cell Analysis System, a platform that allows high-throughput capture of nucleic acids from single cells using a simple cartridge workflow and a multitier barcoding system. The resulting captured information can be used to generate various types of next-generation sequencing (NGS) libraries, including whole transcriptome analysis for discovery biology and targeted RNA analysis for high sensitivity transcript detection. The BD Rhapsody system can be used with emerging applications, such as BD™ AbSeq assays, to profile gene expression in both mRNA and protein level to provide ultra-high resolution analysis of single cells.Keywords: Antibody; Barcoded beads; Magnetic beads; Microwell array; Molecular barcoding; Next-generation sequencing; Single cells; Targeted RNA-seq; Transcriptome; UMI; Unique molecular identifiers
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Year: 2019 PMID： 30968361 DOI： 10.1007/978-981-13-6037-4_5
Source DB: PubMed Journal: Adv Exp Med Biol ISSN： 0065-2598 Impact factor: 2.622