| Literature DB >> 30962512 |
Hongbo Li1, Yingzhu Li1, Qiong Zhao2, Tingting Li1, Juan Wei1, Baiying Li2, Wenjin Shen1, Chao Yang1, Yonglun Zeng2, Pedro L Rodriguez3, Yunde Zhao4, Liwen Jiang5, Xiaojing Wang6, Caiji Gao7.
Abstract
The endosomal sorting complex required for transport (ESCRT) machinery has been well documented for its function in endosomal sorting in eukaryotes. Here, we demonstrate an up-to-now unknown and non-endosomal function of the ESCRT component in plants. We show that FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING 1 (FREE1), a recently identified plant-specific ESCRT component essential for multivesicular body biogenesis, plays additional functions in the nucleus in transcriptional inhibition of abscisic acid (ABA) signalling. Following ABA treatment, SNF1-related protein kinase 2 (SnRK2) kinases phosphorylate FREE1, a step requisite for ABA-induced FREE1 nuclear import. In the nucleus, FREE1 interacts with the basic leucine zipper transcription factors ABA-RESPONSIVE ELEMENTS BINDING FACTOR4 and ABA-INSENSITIVE5 to reduce their binding to the cis-regulatory sequences of downstream genes. Collectively, our study demonstrates the crosstalk between endomembrane trafficking and ABA signalling at the transcriptional level and highlights the moonlighting properties of the plant ESCRT subunit FREE1, which has evolved unique non-endosomal functions in the nucleus besides its roles in membrane trafficking in the cytoplasm.Entities:
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Year: 2019 PMID： 30962512 DOI： 10.1038/s41477-019-0400-5
Source DB: PubMed Journal: Nat Plants ISSN： 2055-0278 Impact factor: 15.793