Nikolaos Papagiannakis1, Maria Xilouri2, Christos Koros3, Athina-Maria Simitsi3, Maria Stamelou3, Matina Maniati2, Leonidas Stefanis4. 1. Center of Clinical Research, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece; 1st Department of Neurology, Eginition Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece. 2. Center of Clinical Research, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece. 3. 1st Department of Neurology, Eginition Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece. 4. Center of Clinical Research, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece; 1st Department of Neurology, Eginition Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece. Electronic address: lstefanis@bioacademy.gr.
Abstract
BACKGROUND: Alpha-synuclein aggregation is considered one of the main causes of Parkinson's Disease (PD). Malfunction of autophagy-lysosomal pathways is believed to be an underlying mechanism of α-synuclein aggregation. Although such malfunction has been observed in PD brains, it is unclear whether it may also occur in extraneuronal tissues. OBJECTIVES: To assess lysosome-mediated protein degradation in cultured Peripheral Blood Mononuclear Cells (PBMCs) of PD patients and healthy controls. METHODS: Total protein degradation in cultured PBMCs was measured by labelling the cells with 3H-leucine using pulse-chase experiments. Different inhibitors were used to measure a range of autophagic pathways. RESULTS: Protein degradation through the main autophagic pathways is reduced in PD patients (n = 18) compared to age- and sex-matched healthy controls (n = 18), (macroautophagy, p = .018; Chaperone-Mediated autophagy, p = .04; and total lysosomal function, p = .007). CONCLUSIONS: Lysosomal dysfunction is present in cultured PBMCs of PD patients, suggesting that it may reflect a systemic feature of the disease.
BACKGROUND:Alpha-synuclein aggregation is considered one of the main causes of Parkinson's Disease (PD). Malfunction of autophagy-lysosomal pathways is believed to be an underlying mechanism of α-synuclein aggregation. Although such malfunction has been observed in PD brains, it is unclear whether it may also occur in extraneuronal tissues. OBJECTIVES: To assess lysosome-mediated protein degradation in cultured Peripheral Blood Mononuclear Cells (PBMCs) of PDpatients and healthy controls. METHODS: Total protein degradation in cultured PBMCs was measured by labelling the cells with 3H-leucine using pulse-chase experiments. Different inhibitors were used to measure a range of autophagic pathways. RESULTS: Protein degradation through the main autophagic pathways is reduced in PDpatients (n = 18) compared to age- and sex-matched healthy controls (n = 18), (macroautophagy, p = .018; Chaperone-Mediated autophagy, p = .04; and total lysosomal function, p = .007). CONCLUSIONS:Lysosomal dysfunction is present in cultured PBMCs of PDpatients, suggesting that it may reflect a systemic feature of the disease.
Authors: Matthew C Deen; Yanping Zhu; Christina Gros; Na Na; Pierre-André Gilormini; David L Shen; Sandeep Bhosale; Nadia Anastasi; RuiQi Wang; Xiaoyang Shan; Eva Harde; Ravi Jagasia; Francis C Lynn; David J Vocadlo Journal: Proc Natl Acad Sci U S A Date: 2022-07-12 Impact factor: 12.779
Authors: Julia D Vavilova; Anna A Boyko; Natalya I Troyanova; Natalya V Ponomareva; Vitaly F Fokin; Ekaterina Y Fedotova; Maria A Streltsova; Sofya A Kust; Maria V Grechikhina; Olga A Shustova; Tatyana L Azhikina; Elena I Kovalenko; Alexander M Sapozhnikov Journal: Biomolecules Date: 2022-03-24